p73 Antibody (ST05-76)

Western Blot: p73 Antibody (ST05-76) [NBP2-67549] - Western blot analysis of p73 on 293 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit

Immunocytochemistry/Immunofluorescence: p73 Antibody (ST05-76) [NBP2-67549] - Staining p73 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: p73 Antibody (ST05-76) [NBP2-67549] - Analysis of paraffin-embedded mouse skin tissue using anti-p73 antibody. Counter stained with hematoxylin.

Western Blot: p73 Antibody (ST05-76) [NBP2-67549] - Analysis of p73 on Hela cell lysates using anti-p73 antibody at 1/1,000 dilution.

Immunohistochemistry-Paraffin: p73 Antibody (ST05-76) [NBP2-67549] - Analysis of paraffin-embedded human kidney tissue using anti-p73 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: p73 Antibody (ST05-76) [NBP2-67549] - Analysis of paraffin-embedded mouse kidney tissue using anti-p73 antibody. Counter stained with hematoxylin.

Product Discontinued

p73 Antibody (ST05-76) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Recombinant protein within Human p73 aa 6-118 / 636. (SwissProt: O15350 Human; SwissProt: Q9JJP2 Mouse)
• Localization: Cytoplasm, Nucleus.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: TP73
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for p73 Antibody (ST05-76)

• p53-related protein
• P73p53-like transcription factor
• TA p73
• TAp73
• tumor protein p73

Background

p73 is a transcription factor that shares a significant amino acid identity with p53 and p63 (1). p73 and p63 can both activate p53-responsive genes and induce apoptosis upon over expression (2) Unlike p53, p73 is not induced by DNA damage and is not a target for inactivation by viral oncoproteins (3). Similar to p63, p73 has an additional coding region in the C-terminus (not found in p53) that can undergo complex alternative splicing giving rise to 3 splice forms (alpha, beta, and gamma) (4). Activation of p73 requires the presence of functional c-Abl. It has also be found that mutant p53 can bind to and inactivate p73 (5).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TP73