p70 S6 Kinase/S6K Antibody

Product Discontinued

p70 S6 Kinase/S6K Antibody Summary

• Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the carboxy terminal end of human p70 S6 Kinase protein.
• Specificity: This antibody is directed against the human p70S6K protein. A BLAST analysis was used to suggest cross reactivity with p70S6K proteins.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RPS6KB1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:5000
  • Immunoprecipitation 5 ug/mg
  • Western Blot 1:1000
• Application Notes: This affinity purified antibody has been tested for use in ELISA, immunoprecipitation and western blot. Expect a predominant band approximately 70 kDa in size corresponding - p70S6K by western blotting in the appropriate cell lysate or extract.

Reactivity Notes

Cross reactivity with p70 S6 Kinase/S6K from other sources has not been tested.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 0.1 M Tris Chloride, 0.5 M Sodium Chloride, pH 8.0
• Preservative: 0.01% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for p70 S6 Kinase/S6K Antibody

• EC 2.7.11
• EC 2.7.11.1
• p70 ribosomal S6 kinase alpha
• p70 S6 kinase alpha
• p70 S6 Kinase
• p70 S6 kinase, alpha 1,70 kDa ribosomal protein S6 kinase 1
• p70 S6 kinase, alpha 2
• p70 S6KA
• p70 S6K-alpha
• p70(S6K)-alpha
• p70-alpha
• p70-S6K
• P70S6K1
• PS6K
• ribosomal protein S6 kinase beta-1
• Ribosomal protein S6 kinase I
• ribosomal protein S6 kinase, 70kD, polypeptide 1
• ribosomal protein S6 kinase, 70kDa, polypeptide 1
• RPS6KB1
• S6K
• S6K1
• S6K1p70-S6K 1
• S6K-beta-1
• serine/threonine kinase 14 alpha
• Serine/threonine-protein kinase 14A
• STK14A

Background

The protein p70 S6 kinase is critical for cell cycle progression and cell survival. In response to mitogen stimulation, p70 S6 kinase activation up-regulates ribosomal biosynthesis and enhances the translational capacity. p70S6K phosphorylates the S6 protein of the 40S subunit of the ribosome. This kinase was first characterized as an insulin/mitogen-activated protein kinase, whose major known substrate is the 40S ribosomal subunit protein S6. p70S6K is activated by diverse stimuli through multi-site phosphorylation at positions such as Thr-252 and Ser-434. In Alzheimer's Disease, p70S6K activation is associated with PHF-tau (hyperphosphorylated tau) accumulation. In non-neuronal cells, p70S6K has been shown to regulate actin polymerization.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RPS6KB1
• Uniprot:
  • P23443(Human)