p67phox/NOXA2 Overexpression Lysate (Native)


Western Blot: NOXA2 Overexpression Lysate (Adult Normal) [NBL1-13510] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for NOXA2.

Product Details

Reactivity HuSpecies Glossary
Applications WB

Order Details

p67phox/NOXA2 Overexpression Lysate (Native) Summary

The lysate was created in HEK293T cells, using plasmid ID RC200704 and based on accession number NM_000433. The protein contains a C-terminal DDK tag.
Homo sapiens neutrophil cytosolic factor 2 (NCF2), transcript variant 1, mRNA.


Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
59.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Store at -80C. Avoid freeze-thaw cycles.
RIPA buffer

Lysate Details for p67phox/NOXA2

Protein State


HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for p67phox/NOXA2 Overexpression Lysate (Native)

  • autosomal 2
  • NADPH oxidase activator 2
  • NCF2
  • NCF-2
  • neutrophil cytosol factor 2
  • neutrophil cytosolic factor 2 (65kD, chronic granulomatous disease, autosomal2)
  • neutrophil cytosolic factor 2
  • NOXA2
  • NOXA2FLJ93058
  • p67phox
  • p67-phox


This gene encodes neutrophil cytosolic factor 2, the 67-kilodalton cytosolic subunit of the multi-protein NADPH oxidase complex found in neutrophils. This oxidase produces a burst of superoxide which is delivered to the lumen of the neutrophil phagosome. Mutations in this gene, as well as in other NADPH oxidase subunits, can result in chronic granulomatous disease, a disease that causes recurrent infections by catalase-positive organisms. Alternative splicing results in two transcript variants that encode the same protein. [provided by RefSeq]. Transcript Variant: This variant (1) represents the longer transcript. Both variants 1 and 2 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for p67phox/NOXA2 Lysate (NBL1-13510) (0)

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Gene Symbol NCF2