p38 gamma/SAPK3 Antibody

Western Blot: p38 gamma/SAPK3 Antibody [H00006300-D01P] - Analysis of MAPK12 expression in transfected 293T cell line by MAPK12 polyclonal antibody.Lane 1: MAPK12 transfected lysate(41.90 KDa).Lane 2: Non-transfected lysate.

Proximity Ligation Assay: p38 gamma/SAPK3 Antibody [H00006300-D01P] - Analysis of protein-protein interactions between MAPK12 and DLG1. HeLa cells were stained with anti-MAPK12 rabbit purified polyclonal 1:1200 and anti-DLG1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Product Discontinued

p38 gamma/SAPK3 Antibody Summary

• Description: Quality control test: Antibody reactive against mammalian transfected lysate.
• Immunogen: MAPK12 (NP_002960.2, 1 a.a. - 367 a.a.) full-length human protein. MSSPPPARSGFYRQEVTKTAWEVRAVYRDLQPVGSGAYGAVCSAVDGRTGAKVAIKKLYRPFQSELFAKRAYRELRLLKHMRHENVIGLLDVFTPDETLDDFTDFYLVMPFMGTDLGKLMKHEKLGEDRIQFLVYQMLKGLRYIHAAGIIHRDLKPGNLAVNEDCELKILDFGLARQADSEMTGYVVTRWYRAPEVILNWMRYTQTVDIWSVGCIMAEMITGKTLFKGSDHLDQLKEIMKVTGTPPAEFVQRLQSDEAKNYMKGLPELEKKDFASILTNASPLAVNLLEKMLVLDAEQRVTAGEALAHPYFESLHDTEDEPQVQKYDDSFDDVDRTLDEWKRVTYKEVLSFKPPRQLGARVSKETPL
• Specificity: MAPK12 - mitogen-activated protein kinase 12,
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MAPK12
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Proximity Ligation Assay
  • Western Blot
• Application Notes: Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB and also on transfected lysate in WB. GST tag alone is used as a negative control.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: No Preservative
• Purity: Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for p38 gamma/SAPK3 Antibody

• EC 2.7.11
• ERK3
• ERK-6
• ERK6MAPK 12
• Extracellular signal-regulated kinase 6
• MAP kinase 12
• MAP kinase p38 gamma
• MAPK12
• mitogen-activated protein kinase 12
• mitogen-activated protein kinase 3
• Mitogen-activated protein kinase p38 gamma
• p38 gamma
• p38gamma
• PRKM12
• SAPK-3
• SAPK3EC 2.7.11.24
• Stress-activated protein kinase 3

Background

Activation of members of the mitogen-activated protein kinase family is a major mechanism for transduction of extracellular signals. Stress-activated protein kinases are one subclass of MAP kinases. The protein encoded by this gene functions as a signal transducer during differentiation of myoblasts to myotubes. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for p38 gamma/SAPK3 Antibody (H00006300-D01P)(1)

Publication using H00006300-D01P

• Liu CH, Chen TC, Chau GY et al. An analysis of protein-protein interactions in cross-talk pathways reveals CRKL as a novel prognostic marker in hepatocellular carcinoma. Mol Cell Proteomics 2013-02-08 [PMID: 23397142]

Bioinformatics

• Gene Symbol: MAPK12
• Entrez:
  • 6300(Human)
• Uniprot:
  • NP_002960.2(Human)