p38 beta/gamma/delta Antibody (PSH03-59)

Western Blot: p38 beta/gamma/delta Antibody (PSH03-59) [NBP3-33145] - Western blot analysis of p38 beta/gamma/delta on different lysates with Rabbit anti-p38 beta/gamma/delta antibody (NBP3-33145) at 1/2,000 dilution. Lane 1: 293T cell lysate Lane 2: HeLa cell lysate Lane 3: MCF7 cell lysate Lane 4: K-562 cell lysate Lane 5: MDA-MB-231 cell lysate Lane 6: COS-1 cell lysate Lane 7: RAW264.7 cell lysate Lane 8: Neuro-2a cell lysate Lane 9: PC-12 cell lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 42 kDa Observed band size: 42 kDa Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33145) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

• Reactivity: Hu, Mu, Rt, Pm
• Applications: WB
• Clone: PSH03-59
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

p38 beta/gamma/delta Antibody (PSH03-59) Summary

• Description: Novus Biologicals Rabbit p38 beta/gamma/delta Antibody (PSH03-59) (NBP3-33145) is a recombinant monoclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant protein within human MAPK12 aa 1-367 / 367. (Uniprot: Q15759)
• Localization: Cytoplasm, Nucleus, Mitochondrion.
• Specificity: Anti-p38 beta/MAPK11 + p38 gamma/MAPK12 + p38 delta/MAPK13 Antibody [PSH03-59] detects endogenous levels of total p38 beta, p38 gamma and p38 delta MAPK protein. This antibody does not recognize p38 alpha MAPK protein.
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: MAPK11
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:2000
• Theoretical MW: 42 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH7.4), 0.1% BSA and 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for p38 beta/gamma/delta Antibody (PSH03-59)

• MAPK11
• MAPK12
• MAPK13
• p38Beta
• p38delta
• p38gamma

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MAPK11