P2Y14/GPR105 Antibody - BSA Free

Immunohistochemistry-Paraffin: P2Y14/GPR105 Antibody [NBP2-93909] - Immunohistochemistry of paraffin-embedded rat ovary using P2Y14/GPR105 Rabbit pAb (NBP2-93909) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Western Blot: P2Y14/GPR105 Antibody [NBP2-93909] - analysis of K-562, using P2RY14 Rabbit pAb at 1:1500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 30s.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

P2Y14/GPR105 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit P2Y14/GPR105 Antibody - BSA Free (NBP2-93909) is a polyclonal antibody validated for use in IHC, WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 209-338 of human P2RY14 (NP_055694.3). ITKKIFKSHLKSSRNSTSVKKKSSRNIFSIVFVFFVCFVPYHIARIPYTKSQTEAHYSCQSKEILRYMKEFTLLLSAANVCLDPIIYFFLCQPFREILCKKLHIPLKAQNDLDISRIKRGNTTLESTDTL
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: P2RY14
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA Recommended starting concentration is 1 ug/mL
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500 - 1:2000
• Theoretical MW: 38 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for P2Y14/GPR105 Antibody - BSA Free

• G protein coupled receptor for UDP-glucose
• GPR105
• G-protein coupled receptor 105
• KIAA0001G protein-coupled receptor 105
• P2RY14
• P2Y purinoceptor 14
• P2Y(14) receptor
• P2Y14 receptor
• P2Y14
• purinergic receptor P2Y, G-protein coupled, 14
• UDP-glucose receptor

Background

GPR105 is a Sugar-Sensing Receptor. GPR105 is a G-protein-coupled receptor identifying a quiescent, primitive population of hematopoietic cells restricted to bone marrow. GPC105 has been suggested to mediates primitive cell responses to specific hematopoietic microenvironments and extends the known immune system functions of P2Y receptors to the stem cell level. GPR105 has also been suggested to play an important role in peripheral and neuroimmune function in response to extracellular UDP-sugars. GPR105 has been reported to be expressed in brain. ESTs have been isolated from kidney, placenta, uterus, and heart/melanocyte/uterus libraries.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: P2RY14