P2X7/P2RX7 Antibody - BSA Free

Immunohistochemistry-Paraffin: P2X7/P2RX7 Antibody [NBP2-19654] - Human breast cancer, using P2X7 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Immunohistochemistry-Paraffin: P2X7/P2RX7 Antibody [NBP2-19654] - Mouse brain. P2X7 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Immunohistochemistry-Paraffin: P2X7/P2RX7 Antibody [NBP2-19654] - Rat lymph node.P2X7 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

• Reactivity: Hu, Mu, Rt
• Applications: IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

P2X7/P2RX7 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit P2X7/P2RX7 Antibody - BSA Free (NBP2-19654) is a polyclonal antibody validated for use in IHC and WB. Anti-P2X7/P2RX7 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human P2X7/P2RX7. The exact sequence is proprietary.
• Localization: Membrane; Multi-pass membrane protein
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: P2RX7
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
• Theoretical MW: 69 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Canine (89%), Bovine (85%), Guinea pig (81%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for P2X7/P2RX7 Antibody - BSA Free

• ATP receptor
• MGC20089
• P2RX7
• P2X purinoceptor 7
• P2X7
• P2X7P2X7 receptor
• P2X7R
• P2Z receptor
• purinergic receptor P2X, ligand-gated ion channel, 7
• purinergic receptor P2X7 variant A
• Purinergic receptor

Background

The product of this gene belongs to the family of purinoceptors for ATP. This receptor functions as a ligand-gated ion channel and is responsible for ATP-dependent lysis of macrophages through the formation of membrane pores permeable to large molecules. Activation of this nuclear receptor by ATP in the cytoplasm may be a mechanism by which cellular activity can be coupled to changes in gene expression. Multiple alternatively spliced variants which would encode different isoforms have been identified although some fit nonsense-mediated decay (NMD) criteria. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for P2X7/P2RX7 Antibody (NBP2-19654)(1)

Publication using NBP2-19654

• Gilbert SM, Oliphant CJ, Hassan S et al. ATP in the tumour microenvironment drives expression of nfP2X7, a key mediator of cancer cell survival Oncogene 2018-08-07 [PMID: 30087439] (IF/IHC, Human)

Bioinformatics

• Gene Symbol: P2RX7