p27/Kip1 Antibody

Western Blot: p27/Kip1 Antibody [NB100-2816] - Human Prostate lysate (35 ug protein in RIPA buffer). Antibody at 0.1 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: p27/Kip1 Antibody [NB100-2816] - Staining of paraffin embedded Human Spleen. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Immunohistochemistry-Paraffin: p27/Kip1 Antibody [NB100-2816] - Staining of paraffin embedded Human Prostate. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu, Mu, Rt, Po, Bv, Ca, Fe
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

p27/Kip1 Antibody Summary

• Description: Novus Biologicals Goat p27/Kip1 Antibody (NB100-2816) is a polyclonal antibody validated for use in IHC, WB and ELISA. Anti-p27/Kip1 Antibody: Cited in 3 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-KRPATDDSSTQNK corresponding to internal region according to NP_004055.1.
• Predicted Species: Mouse (100%), Rat (100%), Porcine (100%), Bovine (100%), Feline (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CDKN1B
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5 ug/mL
  • Immunohistochemistry-Paraffin 5 ug/mL
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.1 - 0.3 ug/mL
• Application Notes: Western blot: Approx 26 kDa band observed in lysates of Human Prostate and of Hepatoblastoma cell line HepG2 (calculated MW of 22.1 kDa according to NP_004055.1). ).
• Control:

  Human Prostate Whole Tissue Lysate (Adult Whole Normal)

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for p27/Kip1 Antibody

• CDKN1B
• CDKN4
• cyclin-dependent kinase inhibitor 1B (p27, Kip1)
• cyclin-dependent kinase inhibitor 1B
• Cyclin-dependent kinase inhibitor p27
• Kip1
• KIP1P27KIP1
• MEN1B
• MEN4
• p27
• p27Kip1

Background

Cell cycle progression is regulated by cyclins and their cognate Cdks. p27 KIP 1 is a cell cycle regulatory mitotic inhibitor of cdk activity. p27 KIP 1 is a candidate tumor suppressor gene, and has been proposed to function as a possible mediator of TGF beta induced G1 arrest. p27 KIP 1 is up regulated in response to antimitogenic stimuli. The increased protein expression of p27 results in cellular arrest by binding to cyclin/Cdk complexes such as cyclin D1/Cdk4. p27 Kip1 is regulated by phosphorylation on serine 10 (S10) and threonine 187 (T187). Phosphorylation by CDK2 on T187 results in ubiquitylation and degradation of p27 Kip 1; while phosphorylation by hKIS on S10 signals the nuclear export to the cytoplasm.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for p27/Kip1 Antibody (NB100-2816)(3)

Publications using NB100-2816

• Beharry Z, Zemskova M, Mahajan S et al. Novel benzylidene-thiazolidine-2,4-diones inhibit Pim protein kinase activity induce cell cycle arrest in leukemia prostate cancer cells. Mol Cancer Ther;8(6):1473-1483. 2009-01-01 [PMID: 19509254]
• Xia ZB, Popovic R, Chen J et al. The MLL fusion gene, MLL-AF4, regulates cyclin-dependent kinase inhibitor CDKN1B (p27kip1) expression. Proc Natl Acad Sci USA 2005-09-01 [PMID: 16169901]
• Baldassarre G, Belletti B, Nicoloso MS et al. p27(Kip1)-stathmin interaction influences sarcoma cell migration and invasion. Cancer Cell 2005-01-01 [PMID: 15652749]

Bioinformatics

• Gene Symbol: CDKN1B
• Entrez:
  • 1027(Human)
• Uniprot:
  • NP_004055.1(Human)