p27/Kip1 Antibody (DCS-72.F6)

Images

 
Immunohistochemistry-Paraffin: p27/Kip1 Antibody (DCS-72.F6) [NBP2-44496] - Human Cervical Cancer stained with p27 Monoclonal Antibody (DCS-72.F6)
Flow Cytometry: p27/Kip1 Antibody (DCS-72.F6) [NBP2-44496] - Human p27 on HeLa Cells. Black: Cells alone; Grey: Isotype Control; Green: AF488-labeled p27 Monoclonal Antibody (DCS-72.F6).
Immunohistochemistry-Paraffin: p27/Kip1 Antibody (DCS-72.F6) [NBP2-44496] - Human Colon Carcinoma stained with p27 Monoclonal Antibody (DCS-72.F6)

Product Details

Summary
Reactivity Hu, Mu, Rt, PmSpecies Glossary
Applications WB, Flow, IHC, IHC-P, ICC/IF
Clone
DCS-72.F6
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

p27/Kip1 Antibody (DCS-72.F6) Summary

Immunogen
Mouse recombinant p27 protein
Localization
Nuclear
Specificity
Recognizes a 27kDa protein, identified as the p27Kip1, a cell cycle regulatory mitotic inhibitor. Its epitope spans between aa 83-204 of p27. It is highly specific and shows no cross-reaction with other related mitotic inhibitors. p27Kip1 functions as a negative regulator of G1 progression and has been proposed to function as a possible mediator of TGF- induced G1 arrest. p27Kip1 is a candidate tumor suppressor gene. This MAb co-precipitates cdk4 in complex p27Kip1 and is excellent for staining of formalin-fixed tissues.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CDKN1B
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5 - 1.0 ug/ml
  • Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 0.25 - 0.5 ug/ml
  • Immunofluorescence 0.5 - 1.0 ug/ml
Application Notes
Hu-chromosome location: 12p13.1
Immunohistochemistry-Paraffin 0.25 - 0.5 ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10 - 20 min followed by cooling at RT for 20 minutes. The 7mL size is a pre-diluted size and no additional dilutions are required before using this item for the intended application.
Theoretical MW
25.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
p27/Kip1 Knockout HeLa Cell Lysate

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Alternate Names for p27/Kip1 Antibody (DCS-72.F6)

  • CDKN1B
  • CDKN4
  • cyclin-dependent kinase inhibitor 1B (p27, Kip1)
  • cyclin-dependent kinase inhibitor 1B
  • Cyclin-dependent kinase inhibitor p27
  • Kip1
  • KIP1P27KIP1
  • MEN1B
  • MEN4
  • p27
  • p27Kip1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-47770AF350
Alexa Fluor 405 NBP2-47770AF405
Alexa Fluor 488 NBP2-47770AF488
Alexa Fluor 532 NBP2-47770AF532
Alexa Fluor 594 NBP2-47770AF594
Alexa Fluor 647 NBP2-47770AF647
Alexa Fluor 700 NBP2-47770AF700
Alexa Fluor 750 NBP2-47770AF750
Allophycocyanin NBP2-47770APC
Biotin NBP2-47770B
DyLight 350 NBP2-47770UV
DyLight 405 NBP2-47770V
DyLight 488 NBP2-47770G
DyLight 550 NBP2-47770R
DyLight 594 NBP2-47770DL594
DyLight 650 NBP2-47770C
DyLight 680 NBP2-47770FR
DyLight 755 NBP2-47770IR
FITC NBP2-47770F
HRP NBP2-47770H
Janelia Fluor 549 NBP2-47770JF549
Janelia Fluor 646 NBP2-47770JF646
PE NBP2-47770PE
PerCP NBP2-47770PCP

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Bioinformatics

Gene Symbol CDKN1B
Uniprot