Reactivity | Hu, Mu, Rt, PmSpecies Glossary |
Applications | WB, Flow, IHC, IHC-P, ICC/IF |
Clone | DCS-72.F6 |
Clonality | Monoclonal |
Host | Mouse |
Conjugate | Unconjugated |
Concentration | 0.2 mg/ml |
Immunogen | Mouse recombinant p27 protein |
Localization | Nuclear |
Specificity | Recognizes a 27kDa protein, identified as the p27Kip1, a cell cycle regulatory mitotic inhibitor. Its epitope spans between aa 83-204 of p27. It is highly specific and shows no cross-reaction with other related mitotic inhibitors. p27Kip1 functions as a negative regulator of G1 progression and has been proposed to function as a possible mediator of TGF- induced G1 arrest. p27Kip1 is a candidate tumor suppressor gene. This MAb co-precipitates cdk4 in complex p27Kip1 and is excellent for staining of formalin-fixed tissues. |
Isotype | IgG1 Kappa |
Clonality | Monoclonal |
Host | Mouse |
Gene | CDKN1B |
Purity | Protein A or G purified |
Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
Dilutions |
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Application Notes | Hu-chromosome location: 12p13.1 Immunohistochemistry-Paraffin 0.25 - 0.5 ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10 - 20 min followed by cooling at RT for 20 minutes. The 7mL size is a pre-diluted size and no additional dilutions are required before using this item for the intended application. |
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Theoretical MW | 25.5 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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Control |
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Storage | Store at 4C. |
Buffer | 10 mM PBS with 0.05% BSA |
Preservative | 0.05% Sodium Azide |
Concentration | 0.2 mg/ml |
Purity | Protein A or G purified |
Secondary Antibodies |
Isotype Controls |
Alexa Fluor 350 | NBP2-47770AF350 | |
Alexa Fluor 405 | NBP2-47770AF405 | |
Alexa Fluor 488 | NBP2-47770AF488 | |
Alexa Fluor 532 | NBP2-47770AF532 | |
Alexa Fluor 594 | NBP2-47770AF594 | |
Alexa Fluor 647 | NBP2-47770AF647 | |
Alexa Fluor 700 | NBP2-47770AF700 | |
Alexa Fluor 750 | NBP2-47770AF750 | |
Allophycocyanin | NBP2-47770APC | |
Biotin | NBP2-47770B | |
DyLight 350 | NBP2-47770UV | |
DyLight 405 | NBP2-47770V | |
DyLight 488 | NBP2-47770G | |
DyLight 550 | NBP2-47770R | |
DyLight 594 | NBP2-47770DL594 | |
DyLight 650 | NBP2-47770C | |
DyLight 680 | NBP2-47770FR | |
DyLight 755 | NBP2-47770IR | |
FITC | NBP2-47770F | |
HRP | NBP2-47770H | |
Janelia Fluor 549 | NBP2-47770JF549 | |
Janelia Fluor 646 | NBP2-47770JF646 | |
PE | NBP2-47770PE | |
PerCP | NBP2-47770PCP |
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