p27/Kip1 Antibody (4B4-E6) - Azide and BSA Free

Western Blot: p27/Kip1 Antibody (4B4-E6) [H00001027-M01] - Analysis of CDKN1B expression in transfected 293T cell line by CDKN1B monoclonal antibody (M01), clone 4B4-E6.Lane 1: CDKN1B transfected lysate(22.1 KDa).Lane 2: Non-transfected lysate.

Immunocytochemistry/Immunofluorescence: p27/Kip1 Antibody (4B4-E6) [H00001027-M01] - Analysis of monoclonal antibody to CDKN1B on HeLa cell. Antibody concentration 10 ug/ml.

Immunohistochemistry-Paraffin: p27/Kip1 Antibody (4B4-E6) [H00001027-M01] - Analysis of monoclonal antibody to CDKN1B on formalin-fixed paraffin-embedded human ovary, clear cell carcinoma tissue. Antibody concentration 5 ug/ml.

Western Blot: p27/Kip1 Antibody (4B4-E6) [H00001027-M01] - Western Blot analysis of CDKN1B expression in HL-60 ( Cat # L014V1 ).

Proximity Ligation Assay: p27/Kip1 Antibody (4B4-E6) [H00001027-M01] - Analysis of protein-protein interactions between AKT1 and CDKN1B. HeLa cells were stained with anti-AKT1 rabbit purified polyclonal 1:1200 and anti-CDKN1B mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Sandwich ELISA: p27/Kip1 Antibody (4B4-E6) [H00001027-M01] - Detection limit for recombinant GST tagged CDKN1B is 10 ng/ml as a capture antibody.

Sandwich ELISA: p27/Kip1 Antibody (4B4-E6) [H00001027-M01] - Detection limit for recombinant GST tagged CDKN1B is 0.03 ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, Func, ICC/IF, IHC
• Clone: 4B4-E6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

p27/Kip1 Antibody (4B4-E6) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: CDKN1B (AAH01971, 1 a.a. ~ 198 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MSNVRVSNGSPSLERMDARQAEHPKPSACRNLFGPVDHEELTRDLEKHCRDMEEASQRKWNFDFQNHKPLEGKYEWQEVEKGSLPEFYYRPPRPPKGACKVPAQESQDGSGSRPAAPLIGAPANSEDTHLVDPKTDPSDSQTGLAEQCAGIRKRPATGDSSTQNKRANRTEENVSDGSPNAGSVEQTPKKPGLRRRQT
• Specificity: CDKN1B - cyclin-dependent kinase inhibitor 1B (p27, Kip1)
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: CDKN1B
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Proximity Ligation Assay
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactivity against recominant protein and cell lysate for WB. It has also been used for IF, IHC-P and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for p27/Kip1 Antibody (4B4-E6) - Azide and BSA Free

• CDKN1B
• CDKN4
• cyclin-dependent kinase inhibitor 1B (p27, Kip1)
• cyclin-dependent kinase inhibitor 1B
• Cyclin-dependent kinase inhibitor p27
• Kip1
• KIP1P27KIP1
• MEN1B
• MEN4
• p27
• p27Kip1

Background

This gene encodes a cyclin-dependent kinase inhibitor, which shares a limited similarity with CDK inhibitor CDKN1A/p21. The encoded protein binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for p27/Kip1 Antibody (H00001027-M01)(1)

Publication using H00001027-M01

• Massimi M, Cardarelli S, Galli F et al. Increase of Intracellular Cyclic Amp by PDE4 Inhibitors Affects HepG2 Cell Cycle Progression and Survival. J Cell Biochem 2016-11-16 [PMID: 27859531]

Bioinformatics

• Gene Symbol: CDKN1B
• Entrez:
  • 1027(Human)
• OMIM:
  • 600778(Human)
• Uniprot:
  • AAH01971(Human)