Rat p21/CIP1/CDKN1A ELISA Kit (Colorimetric)

ELISA: Rat p21/CIP1/CDKN1A ELISA Kit (Colorimetric) [NBP2-75207] - Samples were spiked with high concentrations of Rat p21/CIP1/CDKN1A and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Rat p21/CIP1/CDKN1A ELISA Kit (Colorimetric) [NBP2-75207] - Standard Curve Reference

• Reactivity: Rt
• Applications: ELISA
• Suitable Sample Type: Serum, plasma and other biological fluids
• Standard Curve Range: 78.13 - 5000 pg/mL
• Sensitivity: 46.88 pg/mL

Rat p21/CIP1/CDKN1A ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Rat CDKN1A in samples. No significant cross-reactivity or interference between Rat CDKN1A and analogues was observed.
• Standard Curve Range: 78.13 - 5000 pg/mL
• Sensitivity: 46.88 pg/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: CV% < 4.92%
• Intra-Assay: CV% < 5%
• Spike Recovery: 90-107%
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: CDKN1A

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Rat p21/CIP1/CDKN1A ELISA Kit (Colorimetric)

• CAP20cyclin-dependent kinase inhibitor 1
• CDK-interacting protein 1
• CDKN1A
• CDKN1melanoma differentiation associated protein 6
• CIP1
• CIP1WAF1CDK-interaction protein 1
• cyclin-dependent kinase inhibitor 1A (p21, Cip1)
• MDA6
• MDA-6
• Melanoma differentiation-associated protein 6
• p21
• p21CIP1
• p21Cip1/Waf1
• PIC1
• SDI1DNA synthesis inhibitor
• wild-type p53-activated fragment 1

Background

Cell cycle progression is regulated by cyclins and their cognate Cdks. p21 (WAF1/CIP1) inhibits the activity of each member of the cyclin/Cdk family, and over expression of this protein inhibits the proliferation of mammalian cells. The expression of p21 is inducible by a wide range of stress stimuli. p21 is tightly regulated at the transcriptional level by p53, and probably serves as the effector of p53 cell cycle control.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for p21/CIP1/CDKN1A ELISA Kit (NBP2-75207)(1)

Publication using NBP2-75207

• Hafez Hm, Abdel-Hakeem Ea, Hassanein H Rupatadine, a dual antagonist of histamine and platelet-activating factor (PAF), attenuates experimentally induced diabetic nephropathy in rats Naunyn Schmiedebergs Arch. Pharmacol. 2020-03-21 [PMID: 32200462]

FAQs for p21/CIP1/CDKN1A ELISA Kit (NBP2-75207). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: CDKN1A