p19ARF Antibody (12-A1-1)

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Western Blot: p19ARF Antibody (12-A1-1) [NB200-169] - Analysis of endogenous p19Arf using NB200-169. Total cell lysates (25 ug) from NIH3T3 cells, which have deleted the Arf gene (Lanes 1) and from wild type mouse ...read more
Immunocytochemistry/ Immunofluorescence: p19ARF Antibody (12-A1-1) [NB200-169] - Immunofluorescent detection of p19Arf in wild type mouse embryo fibroblasts (MEF) at passage 5 (lanes 1, 2) and NIH3T3 cells, which have ...read more
Immunoprecipitation: p19ARF Antibody (12-A1-1) [NB200-169] - Total cell lysates (250 ug) from NIH3T3 cells which have deleted the Arf gene (Lanes 5) and from NIH3T3 cells expressing an HA-tagged version of p19Arf (lanes ...read more

Product Details

Summary
Reactivity Mu, Ha(-), Hu(-)Species Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Clone
12-A1-1
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated

Order Details

p19ARF Antibody (12-A1-1) Summary

Immunogen
A synthetic peptide made to a sequence (within residues 1-100) of mouse p19ARF. [UniProt# Q64364]
Epitope
The epitope recognized is between amino acids 54-62.
Specificity
This antibody is specific for murine p19ARF. It does not recognize human p14Arf nor Golden hamster p13Arf.
Isotype
IgG2b
Clonality
Monoclonal
Host
Rat
Gene
CDKN2A
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • ELISA 1:5000-1:10000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:100
Application Notes
This p19ARF (12-A1-1) antibody is useful in ELISA, Immunocytochemistry/Immunofluorescence, Immunohistochemistry on frozen and paraffin-embedded sections, Immunoprecipitation and Western Blot. By Western blot, this antibody recognizes bands at ~19 kDa.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
19 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NB200-169 in the following applications:

  • IHC
    1 publication
  • WB
    2 publications

Reactivity Notes

Mouse. It does not react with human or Golden Syrian hamster. Armenian hamster has not been tested.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
0.1% Sodium Azide
Purity
Unpurified

Alternate Names for p19ARF Antibody (12-A1-1)

  • ARF
  • CDK4 inhibitor p16-INK4
  • CDK4IP14ARF
  • CDKN2
  • cell cycle negative regulator beta
  • CMM2P16-INK4A
  • Cyclin-dependent kinase 4 inhibitor A
  • cyclin-dependent kinase inhibitor 2A (melanoma, p16, inhibits CDK4)
  • cyclin-dependent kinase inhibitor 2A
  • INK4
  • INK4A
  • MLMP16INK4
  • MTS-1
  • MTS1P14
  • Multiple tumor suppressor 1
  • p14
  • p14ARF
  • P16
  • p16-INK4
  • P16INK4A
  • p16-INK4a
  • P19
  • P19ARF
  • TP16

Background

ARF (alternative reading frame; p14ARF in human, p19ARF in mouse) is a tumor suppressor that serves as a sensor of hyper-proliferative signals, resulting in p53-dependent growth arrest and apoptotic cell death. ARF interacts with several proteins including COMMD1, CDKN2AIP, TBRG1, E4F1, C1QBP etc. and can bind to BCL6, E2F1, HUWE1, MDM2, MYC, NPM1/B23, TOP1/TOPOI as well as UBE2I/UBC9. ARF has the capability of inducing cell cycle arrest in G1 as well as G2 phases. While ARF is not expressed at significant levels in most normal tissues, oncogene activation triggers ARF overexpression, resulting in inhibition of MDM2 ubiquitin ligase and stabilization of p53. It is induced by progesterone as well as Ras/DMTF1, and is downregulated through NFkB p65 subunit (RELA). Inhibition of the p53 pathway, most commonly via mutations in p53 itself, inactivation of ARF, or amplification of MDM2 has been proposed to be a crucial step in pathogenesis of most human malignancies. Moreover, mice with null ARF have been shown to develop tumors rapidly and to display delayed mammary gland involution.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for p19ARF Antibody (NB200-169)(4)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol CDKN2A
Entrez
Uniprot