p15INK4b/CDKN2B Recombinant Protein Antigen

• Reactivity: Hu
• Applications: AC

p15INK4b/CDKN2B Recombinant Protein Antigen Summary

• Description: A recombinant protein antigen with a N-terminal His6-ABP tag corresponding to human p15INK4b/CDKN2B
  
  

  Source: E.coli

  Amino Acid Sequence: REGFLDTLVVLHRAGARLDVRDAWGRLPVDLAEE

  Fusion Tag: N-terminal His6ABP (ABP = Albumin Binding Protein derived from Streptococcal Protein G)

• Protein/Peptide Type: Recombinant Protein Antigen
• Gene: CDKN2B
• Purity: >80% by SDS-PAGE and Coomassie blue staining

Applications/Dilutions

• Dilutions:
  • Antibody Competition 10-100 molar excess
• Application Notes: This recombinant antigen is only intended to be used as a blocking agent to confirm antibody specificity with the corresponding antibody, catalog number NBP3-24782It is purified by IMAC chromatography, and the expected concentration is greater than 0.5 mg/ml.For current lot information, including availability, please contact our technical support team click nb-technical@bio-techne.com.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS and 1M Urea, pH 7.4.
• Preservative: No Preservative
• Purity: >80% by SDS-PAGE and Coomassie blue staining

Alternate Names for p15INK4b/CDKN2B Recombinant Protein Antigen

• CDK4I
• CDKN2B
• cyclin-dependent kinase inhibitor 2B (p15, inhibits CDK4)
• cyclin-dependent kinases 4 and 6 binding protein
• INK4BCDK4B inhibitor
• MTS2
• MTS-2
• MTS2CDK inhibitory protein
• Multiple tumor suppressor 2
• p14_CDK inhibitor
• p14_INK4B
• p14-INK4b
• p15 CDK inhibitor
• p15_INK4B
• P15cyclin-dependent kinase 4 inhibitor B
• p15INK4b
• p15-INK4b
• TP15

Background

The normal progression of cells through the cell cycle is under the control of the cyclin dependent protein kinases Cdk4 and Cdk6 which are subject to inhibition by the mitotic inhibitory protein, p16. An isolated member of the p16 family has been designated p15. p15 expression is upregulated approximately 30-fold in TGFbeta-treated human keratinocytes, suggesting that p15 may act as an effector of TGFbeta-mediated cell cycle arrest. The gene encoding p15 has been mapped to chromosome 9p21 at a position adjacent to the p16 gene at a site of frequent chromosomal abnormality in human tumors. It has been suggested that p15 may function as an effector of TGFbeta-mediated cell cycle arrest through inhibition of Cdk4 and Cdk6 kinases.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CDKN2B