p15INK4b/CDKN2B Antibody (651308) [Alexa Fluor® 488]

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clone: 651308
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 488

p15INK4b/CDKN2B Antibody (651308) [Alexa Fluor® 488] Summary

• Immunogen: E. coli-derived recombinant human p15INK4b/CDKN2B
  Arg2-Asp138
  Accession # P42772
• Specificity: Detects human p15INK4b/CDKN2B in direct ELISAs and Western blots. In Western blots, approximately 50% cross-reactivity with recombinant human (rh) p16INK4a and no cross-reactivity with rhp18INK4c or rhp19INK4d is observed.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for p15INK4b/CDKN2B Antibody (651308) [Alexa Fluor® 488]

• CDK4I
• CDKN2B
• cyclin-dependent kinase inhibitor 2B (p15, inhibits CDK4)
• cyclin-dependent kinases 4 and 6 binding protein
• INK4BCDK4B inhibitor
• MTS2
• MTS-2
• MTS2CDK inhibitory protein
• Multiple tumor suppressor 2
• p14_CDK inhibitor
• p14_INK4B
• p14-INK4b
• p15 CDK inhibitor
• p15_INK4B
• P15cyclin-dependent kinase 4 inhibitor B
• p15INK4b
• p15-INK4b
• TP15

Background

p15INK4B is an approximately 15 kDa member of the p16INK4 family. Human p15INK4B is 138 amino acids (aa) in length and contains four ankyrin repeats, a common structural sequence found in members of the INK4 family. The first 50 aa of p15INK4B share 44% sequence identity with p16INK4. Following this is an 81 aa region sharing 97% sequence identity, after which the two proteins diverge. Human p15INK4B shares 83% aa sequence identity with mouse and rat p15INK4B. Functionally, p15INK4B interacts strongly with CDK4 and CDK6 and is a potent inhibitor. p15INK4B can potentially induce cell cycle arrest by acting as an effector of TGF-beta .

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.