p130Cas/BCAR1 Antibody (685718)

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, Neuro-2A mouse neuroblastoma cell line, and L6 rat myoblast cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human p130Cas Monoclonal Antibody (Catalog # MAB5730) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for p130Cas at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clone: 685718
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

p130Cas/BCAR1 Antibody (685718) Summary

• Immunogen: E. coli-derived recombinant human p130Cas
  Met66-Val412
  Accession # P56945
• Specificity: Detects human p130Cas in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human Ajuba is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: BCAR1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for p130Cas/BCAR1 Antibody (685718)

• BCAR1
• breast cancer estrogen resistance 1
• Cas scaffolding protein family member 1
• CAS1
• CASFLJ45059
• CASS1
• CASS1CRKASbreast cancer estrogen resistance protein 1
• CRKAS
• Crk-associated substrate p130Cas
• CRK-associated substrate
• FLJ12176
• p130Cas

Background

p130Cas (protein 130 kDa Crk-associated substrate; also BCAR1) is a cytoplasmic member of the CAS family of proteins. Although its predicted MW is 93 kDa, it runs anomalously at 105-130 kDa in SDS-PAGE. It is found in a wide variety of cell types. p130Cas is involved in cell spreading and migration (but not adhesion), and its cleavage into a 31 kDa fragment promotes anoikis and cell death. Human p130Cas is 870 amino acids (aa) in length. It contains one SH3 domain (aa 3-65), a Pro‑rich region (aa 74-87), at least 10 phosphotyrosine (aa 128-410) and four phosphoserine (aa 134-639) sites, an SH3 binding motif (aa 635-643) and a HLH dimerization segment that interacts with CASL. There are multiple splice variants. There is a 22 aa substitution for aa 1-4, an alternate start site at Met30, a deletion of aa 2-211, and a four aa substitution for aa 1-4 with a deletion of aa 65-212. Over aa 66-412, human p130Cas shares 91% aa identity with mouse p130Cas.    

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for p130Cas/BCAR1 Antibody (MAB5730)(1)

Publication using MAB5730

• Hideki Tsumura, Morihiro Ito, Masamichi Takami, Miyuki Arai, Xiao-Kang Li, Toshio Hamatani et al. Conditional deletion of CD98hc inhibits osteoclast development Biochemistry and Biophysics Reports 2016-03-01 [PMID: 28955825]

Bioinformatics

• Gene Symbol: BCAR1
• Uniprot:
  • P56945()