p130Cas/BCAR1 Antibody (685718) [Alexa Fluor® 594]

• Applications: WB
• Clone: 685718
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 594

p130Cas/BCAR1 Antibody (685718) [Alexa Fluor® 594] Summary

• Immunogen: E. coli-derived recombinant human p130Cas
  Met66-Val412
  Accession # P56945
• Specificity: Detects human p130Cas in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human Ajuba is observed.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

p130Cas (protein 130 kDa Crk-associated substrate; also BCAR1) is a cytoplasmic member of the CAS family of proteins. Although its predicted MW is 93 kDa, it runs anomalously at 105-130 kDa in SDS-PAGE. It is found in a wide variety of cell types. p130Cas is involved in cell spreading and migration (but not adhesion), and its cleavage into a 31 kDa fragment promotes anoikis and cell death. Human p130Cas is 870 amino acids (aa) in length. It contains one SH3 domain (aa 3-65), a Pro‑rich region (aa 74-87), at least 10 phosphotyrosine (aa 128-410) and four phosphoserine (aa 134-639) sites, an SH3 binding motif (aa 635-643) and a HLH dimerization segment that interacts with CASL. There are multiple splice variants. There is a 22 aa substitution for aa 1-4, an alternate start site at Met30, a deletion of aa 2-211, and a four aa substitution for aa 1-4 with a deletion of aa 65-212. Over aa 66-412, human p130Cas shares 91% aa identity with mouse p130Cas.    

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.