p120-catenin [p Ser268] Antibody

Western Blot: p120-catenin [p Ser268] Antibody [NBP3-12923] - Non-transfected (-) and transfected (+) A431 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with p120-catenin (phospho Ser268) antibody (NBP3-12923) diluted at 1:3000.

Western Blot: p120-catenin [p Ser268] Antibody [NBP3-12923] - p120-catenin (phospho Ser268) antibody detects p120-catenin (phospho Ser268) protein by western blot analysis. Un-treated (-) and treated (+, CIP treatment) A431 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with p120-catenin (phospho Ser268) antibody (NBP3-12923) diluted at 1:1000. (CIP: Calf-intestinal alkaline phosphatase)

Western Blot: p120-catenin [p Ser268] Antibody [NBP3-12923] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with p120-catenin (phospho Ser268) antibody (NBP3-12923) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

• Reactivity: Hu, Mu, Rt, Bv, RM
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

p120-catenin [p Ser268] Antibody Summary

• Description: Centrifuge briefly prior to opening.
• Immunogen: Carrier-protein conjugated synthetic peptide surrounding phospho Ser268 of human p120-catenin. The exact sequence is proprietary.
• Modification: p Ser268
• Predicted Species: Rat (100%), Bovine (100%), Rhesus Macaque (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CTNND1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7), 20% Glycerol, 1% BSA
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for p120-catenin [p Ser268] Antibody

• Cadherin-associated Src substrate
• CAS
• catenin (cadherin-associated protein), delta 1
• catenin delta-1
• CTNND
• KIAA0384P120CAS
• p120 catenin
• p120
• p120(CAS)
• p120(CTN)
• p120cas
• p120ctn

Background

The alpha, beta, delta, and gamma -catenins are proteins that bind to the highly conserved, intracellular cytoplasmic tail of E-cadherin. Together, the catenin/cadherin complexes play an important role mediating cellular adhesion. alpha-catenin, a 102 kDa protein, interacts with E-cadherin associated protein, and also associates with other members of the cadherin family, such as N-cadherin and P-cadherin. The 92 kDa beta-catenin associates with the cytoplasmic portion of E-cadherin, which is necessary for the function of E-cadherin as an adhesion molecule. beta-catenin also complexes with the tumor suppressor protein APC. delta-catenin interacts with Presenilin 1 and is expressed in the brain. The gene encoding delta-catenin maps to human chromosome 5p15.2. A hemizygous loss of the gene encoding delta-catenin leads to the mental retardation associated with Cri-du-Chat syndrome. gamma-catenin, also known as plakoglobin, is an 80-88 kDa protein that binds alpha-catenin and N-cadherin. In addition, the transmembrane phosphatase PTPm associates with catenin/cadherin complexes and may regulate complex signaling.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CTNND1