P-Rex1 Antibody

Immunocytochemistry/Immunofluorescence: P-Rex1 Antibody [NBP1-93590] - Staining of human cell line A-431 shows localization to cytosol & vesicles. Antibody staining is shown in green.

Immunohistochemistry-Paraffin: P-Rex1 Antibody [NBP1-93590] - Staining of human lymph node shows moderate cytoplasmic positivity in leukocytes.

Immunohistochemistry-Paraffin: P-Rex1 Antibody [NBP1-93590] - Staining of human small intestine shows moderate cytoplasmic positivity in lymphoid cells.

Immunohistochemistry-Paraffin: P-Rex1 Antibody [NBP1-93590] - Staining of human cerebral cortex shows moderate cytoplasmic positivity in neurons.

Immunohistochemistry-Paraffin: P-Rex1 Antibody [NBP1-93590] - Staining of human hippocampus shows moderate cytoplasmic positivity in neuronal cells.

Immunohistochemistry-Paraffin: P-Rex1 Antibody [NBP1-93590] - Staining of human kidney shows no cytoplasmic positivity as expected.

• Reactivity: Hu
• Applications: ICC/IF, IHC, IHC-P
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

P-Rex1 Antibody Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: CAGQCILKVNGSNVMNDGAPEVLEHFQAFRSRREEALGLYQWIYHTHEDAQEARASQEASTEDPSGEQAQEEDQADSAFPLLSLGPRLSLCEDSPMVTLTVDNVHLEHGVVYEYVSTAGVRCHVLEKIVEP
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PREX1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  P-Rex1 Protein (NBP1-93590PEP)

Reactivity Notes

Reactivity reported in scientific literature (PMID: 23382862)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for P-Rex1 Antibody

• KIAA1415
• phosphatidylinositol 34,5-trisphosphate-dependent Rac exchanger 1 protein
• phosphatidylinositol-34,5-trisphosphate-dependent Rac exchange factor 1
• PRex1
• P-Rex1
• ptdIns(34,5)-dependent Rac exchanger 1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for P-Rex1 Antibody (NBP1-93590)(3)

Publications using NBP1-93590

• Stadler C, Rexhepaj E, Singan VR et al. Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells. Nat Methods 2013-04-01 [PMID: 23435261]
• Campbell AD, Lawn S, McGarry LC et al. P-Rex1 Cooperates with PDGFR-beta to Drive Cellular Migration in 3D Microenvironments. PLoS One 2013-01-01 [PMID: 23382862]
• Lindsay CR, Lawn S, Campbell AD et al. P-Rex1 is required for efficient melanoblast migration and melanoma metastasis. Nat Commun 2011-11-01 [PMID: 22109529]

Bioinformatics

• Gene Symbol: PREX1