Recombinant Human Osteoactivin/GPNMB His Protein

SDS-Page: Recombinant Human Osteoactivin/GPNMB Protein [NBP2-59533] - 15% SDS-PAGE (3ug)

• Reactivity: Hu
• Applications: PAGE
• Concentration: 0.5 mg/ml

Recombinant Human Osteoactivin/GPNMB His Protein Summary

• Description: A recombinant protein with a C-Terminal His-tag and corresponding to the amino acids 22-474 of Human Osteoactivin/GPNMB
  
  

  Source:Baculovirus

  Amino Acid Sequence: ADPAKRFHDV LGNERPSAYM REHNQLNGWS SDENDWNEKL YPVWKRGDMR WKNSWKGGRV QAVLTSDSPA LVGSNITFAV NLIFPRCQKE DANGNIVYEK NCRNEAGLSA DPYVYNWTAW SEDSDGENGT GQSHHNVFPD GKPFPHHPGW RRWNFIYVFH TLGQYFQKLG RCSVRVSVNT ANVTLGPQLM EVTVYRRHGR AYVPIAQVKD VYVVTDQIPV FVTMFQKNDR NSSDETFLKD LPIMFDVLIH DPSHFLNYST INYKWSFGDN TGLFVSTNHT VNHTYVLNGT FSLNLTVKAA APGPCPPPPP PPRPSKPTPS LGPAGDNPLE LSRIPDENCQ INRYGHFQAT ITIVEGILEV NIIQMTDVLM PVPWPESSLI DFVVTCQGSI PTEVCTIISD PTCEITQNTV CSPVDVDEMC LLTVRRTFNG SGTYCVNLTL GDDTSLALTS TLISVPHHHH HH

• Source: Baculovirus
• Protein/Peptide Type: Recombinant Protein
• Gene: GPNMB
• Purity: >90%, by SDS-PAGE
• Endotoxin Note: < 1.0 EU per 1 microgram of protein (determined by LAL method)

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 51.8 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4), 10% glycerol
• Preservative: No Preservative
• Concentration: 0.5 mg/ml
• Purity: >90%, by SDS-PAGE

Alternate Names for Recombinant Human Osteoactivin/GPNMB His Protein

• DC-HIL
• glycoprotein (transmembrane) nmb
• glycoprotein NMB
• GPNMB
• HGFIN
• HGFINglycoprotein nmb-like protein
• NMBosteoactivin
• Osteoactivin
• Transmembrane glycoprotein HGFIN
• transmembrane glycoprotein NMB

Background

The GPNMB gene codes for a type I transmembrane glycoprotein NBM. The GPNMB gene is present in lowly, but not highly, metastic human melanoma cell lines, therefore, it demonstrates involvement in development, proliferation, and differentiation of tissues. Isoform 1 is 572 amino acids long at a mass of nearly 64 kDA and isoform 2 exists at 560 amino acids long at approximately 62 kDA. This gene has been known to interact with various genes such as SMAD4, PLA2G4A, and ITSN2. GPNMB has been researched in its role in retinitis, lung cancer, melanoma, glioblastoma, glaucoma, and breast cancer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: GPNMB