OCTN2/SLC22A5 Overexpression Lysate (Native)

Images

 
Western Blot: SLC22A5 Overexpression Lysate (Adult Normal) [NBL1-16037] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SLC22A5.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

OCTN2/SLC22A5 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC204177 and based on accession number NM_003060. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens solute carrier family 22 (organic cation/carnitine transporter), member 5 (SLC22A5), mRNA.
Gene
SLC22A5

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
62.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for OCTN2/SLC22A5

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for OCTN2/SLC22A5 Overexpression Lysate (Native)

  • CDSP
  • FLJ46769
  • High-affinity sodium-dependent carnitine cotransporter
  • OCTN2
  • OCTN2high-affinity sodium dependent carnitine cotransporter
  • OCTN2VT
  • organic cation transporter 2
  • organic cation transporter 5
  • Organic cation/carnitine transporter 2
  • SCD
  • SLC22A5
  • solute carrier family 22 (organic cation transporter), member 5
  • solute carrier family 22 (organic cation/carnitine transporter), member 5
  • solute carrier family 22 member 5

Background

Polyspecific organic cation transporters in the liver, kidney, intestine, and other organs are critical for elimination of many endogenous small organic cations as well as a wide array of drugs and environmental toxins. The encoded protein is a plasma integral membrane protein which functions both as an organic cation transporter and as a sodium-dependent high affinity carnitine transporter. The encoded protein is involved in the active cellular uptake of carnitine. Mutations in this gene are the cause of systemic primary carnitine deficiency (CDSP), an autosomal recessive disorder manifested early in life by hypoketotic hypoglycemia and acute metabolic decompensation, and later in life by skeletal myopathy or cardiomyopathy. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for OCTN2/SLC22A5 Lysate (NBL1-16037) (0)

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Bioinformatics

Gene Symbol SLC22A5
Entrez