OCRL Antibody

Images

 
Western Blot: OCRL Antibody [NBP2-19621] - Sample (30 ug of whole cell lysate) A: 293T B: A431 5% SDS PAGE gel, diluted at 1:500.
Immunocytochemistry/ Immunofluorescence: OCRL Antibody [NBP2-19621] - A431 cells were fixed in ice-cold MeOH for 5 min. Green: OCRL protein stained by OCRL antibody diluted at 1:500. Blue: Hoechst 33342 staining.
Immunohistochemistry-Paraffin: OCRL Antibody [NBP2-19621] - Immunohistochemical analysis of paraffin-embedded Breast ca, using antibody at 1:250 dilution.
Immunocytochemistry/ Immunofluorescence: OCRL Antibody [NBP2-19621] - Immunofluorescence analysis of methanol-fixed A431, using antibody at 1:500 dilution.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

OCRL Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of human OCRL. The exact sequence is proprietary.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
OCRL
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
104 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Expected cross reactivity based on sequence homology: Rhesus Monkey.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 20% Glycerol
Preservative
0.01% Thimerosal
Purity
Immunogen affinity purified

Alternate Names for OCRL Antibody

  • EC 3.1.3.36
  • INPP5F
  • LOCR
  • Lowe oculocerebrorenal syndrome protein
  • NPHL2
  • OCRL1inositol polyphosphate 5-phosphatase OCRL-1
  • oculocerebrorenal syndrome of Lowe
  • phosphatidylinositol polyphosphate 5-phosphatase

Background

This gene encodes a phosphatase enzyme that is involved in actin polymerization and is found in the trans-Golgi network. Mutations in this gene cause oculocerebrorenal syndrome of Lowe and also Dent disease. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol OCRL
Uniprot