OCRL Antibody - BSA Free

Western Blot: OCRL Antibody [NBP2-19621] - Sample (30 ug of whole cell lysate) A: 293T B: A431 5% SDS PAGE gel, diluted at 1:500.

Immunocytochemistry/Immunofluorescence: OCRL Antibody [NBP2-19621] - A431 cells were fixed in ice-cold MeOH for 5 min. Green: OCRL protein stained by OCRL antibody diluted at 1:500. Blue: Hoechst 33342 staining.

Immunohistochemistry-Paraffin: OCRL Antibody [NBP2-19621] - Human breast cancer, using OCRL antibody at 1:250 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

OCRL antibody detects OCRL protein at cytoplasm by immunofluorescent analysis.Sample: A431 cells were fixed in ice-cold MeOH for 5 min.Green: OCRL protein stained by OCRL antibody (NBP2-19621) diluted at 1:500.Blue: Hoechst 33342 staining.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

OCRL Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit OCRL Antibody - BSA Free (NBP2-19621) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the C-terminus region of human OCRL. The exact sequence is proprietary.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: OCRL
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 104 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rhesus Macaque, Mouse, Rat (80%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris, 0.1M Glycine, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for OCRL Antibody - BSA Free

• EC 3.1.3.36
• INPP5F
• LOCR
• Lowe oculocerebrorenal syndrome protein
• NPHL2
• OCRL1inositol polyphosphate 5-phosphatase OCRL-1
• oculocerebrorenal syndrome of Lowe
• phosphatidylinositol polyphosphate 5-phosphatase

Background

This gene encodes a phosphatase enzyme that is involved in actin polymerization and is found in the trans-Golgi network. Mutations in this gene cause oculocerebrorenal syndrome of Lowe and also Dent disease. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: OCRL
• Uniprot:
  • Q01968(Human)