Recombinant Human NUDT3 His Protein

SDS-Page: Recombinant Human NUDT3 Protein [NBP1-50999] - 15 % SDS-PAGE (3ug)

• Reactivity: Hu
• Applications: PAGE
• Concentration: 0.5 mg/ml

Recombinant Human NUDT3 His Protein Summary

• Description: A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-172 of Human NUDT3
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MMKLKSNQTR TYDGDGYKKR AACLCFRSES EEEVLLVSSS RHPDRWIVPG GGMEPEEEPS VAAVREVCEE AGVKGTLGRL VGIFENQERK HRTYVYVLIV TEVLEDWEDS VNIGRKREWF KIEDAIKVLQ YHKPVQASYF ETLRQGYSAN NGTPVVATTY SVSAQSSMSG IR

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: NUDT3
• Purity: >95%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 21.6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH8.0), 0.2 M NaCl, 5mM DTT, 20% glycerol
• Preservative: No Preservative
• Concentration: 0.5 mg/ml
• Purity: >95%, by SDS-PAGE

Alternate Names for Recombinant Human NUDT3 His Protein

• Diadenosine 5'-5'''-P1
• diphosphoinositol polyphosphate phosphohydrolase 1
• DIPP-1
• DIPPDIPP1
• EC 3.6.1.-
• EC 3.6.1.52
• Nucleoside diphosphate-linked moiety X motif 3
• nudix (nucleoside diphosphate linked moiety X)-type motif 3
• Nudix motif 3
• P6-hexaphosphate hydrolase 1

Background

NUDT3, also known as DIPP, DIPP1 (diphosphoinositol polyphosphate phosphohydrolase 1), is a 172 amino acid cytoplasmic protein belonging to the nudix hydrolase family and DIPP subfamily. NUDT3 acts as a negative regulator of the ERK 1/2 pathway and hydrolyzes 5-phosphoribose 1-diphosphate. NUDT3 exists as a monomer and binds magnesium as a cofactor. Also NUDT3 is widely expressed but found at highest levels in liver, pancreas, brain and heart. Recombinant human NUDT3 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: NUDT3