Recombinant Human NUDT10 His Protein

SDS-Page: Recombinant Human NUDT10 Protein [NBP1-51092] - 15 % SDS-PAGE (3ug)

• Reactivity: Hu
• Applications: PAGE
• Concentration: 0.5 mg/ml

Recombinant Human NUDT10 His Protein Summary

• Description: A recombinant protein with a C-Terminal His-tag and corresponding to the amino acids 1-164 of Human NUDT10
  
  

  Source: E.coli

  Amino Acid Sequence: MKCKPNQTRT YDPEGFKKRA ACLCFRSERE DEVLLVSSSR YPDRWIVPGG GMEPEEEPGG AAVREVYEEA GVKGKLGRLL GVFEQNQDPK HRTYVYVLTV TELLEDWEDS VSIGRKREWF KVEDAIKVLQ CHKPVHAEYL EKLKLGGSPT NGNSMAPSSP DSDPLEHHHH HH

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: NUDT10
• Purity: >95%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 19.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH8.0), 20% glycerol, 0.1 M NaCl, 1 mM DTT
• Preservative: No Preservative
• Concentration: 0.5 mg/ml
• Purity: >95%, by SDS-PAGE

Alternate Names for Recombinant Human NUDT10 His Protein

• APS2DIPP3A
• Diadenosine 5'-5'''-P1
• diphosphoinositol polyphosphate phosphohydrolase 3-alpha
• diphosphoinositol polyphosphate phosphohydrolase type 3 alpha
• DIPP3a
• DIPP-3-alpha
• DIPP3-alpha
• EC 3.6.1.-
• EC 3.6.1.52
• hDIPP3alphahAps2
• Nucleoside diphosphate-linked moiety X motif 10
• nudix (nucleoside diphosphate linked moiety X)-type motif 10
• Nudix motif 10
• P6-hexaphosphate hydrolase 3-alpha

Background

NUDT10, also known as APS2 or DIPP3alpha, is a member of the Nudix hydrolase family of pyrophosphatases. Nudix hydrolases contain a characteristic Nudix domain and are responsible for catalyzing the hydrolysis of nucleoside diphosphate derivatives. It functions as a manganese-dependent polyphosphate phosphohydrolase with an optimum pH of 8.5. This protein specifically metabolizes diadendosine-polyphosphates and, to a lesser extent, diphosphoinositol polyphosphates. Recombinant human NUDT10 protein, fused to His-tag at C-terminus, was expressed in E.coli and purified by using conventional chromatography.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: NUDT10