Nucleoporin NUP85 Antibody (783403) [Alexa Fluor® 594]

• Applications: WB
• Clone: 783403
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 594

Nucleoporin NUP85 Antibody (783403) [Alexa Fluor® 594] Summary

• Immunogen: E. coli-derived recombinant human Nucleoporin NUP85
  Met1-Ser656
  Accession # Q9BW27
• Specificity: Detects human Nucleoporin NUP85 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Nucleoporin NUP85 Antibody (783403) [Alexa Fluor® 594]

• 85 kDa nucleoporin
• FLJ12549
• FROUNT
• nuclear pore complex protein Nup85
• nucleoporin 85kDa
• Nucleoporin Nup75
• Nucleoporin Nup85
• Nucleoporin
• Nup75
• NUP85
• PCNT
• PCNT1
• Pericentrin-1

Background

NUP85 (Nucleoporin 85; also Nup75/nucleoporin 75 and FROUNT) is a 70‑72 kDa member of the nucleoporin Nup85 family of proteins. It is one of nine members of the Nup107-160 subcomplex that performs at least two functions in the cell. First, it serves as an assembly point for nuclear pore formation, and may stabilize the bend that normally exists between the inner and outer nuclear membranes. Second, it appears to be quite stable, and during mitosis, promotes mitotic spindle assembly. Human NUP85 is 656 amino acids (aa) in length. It is an alpha -helical protein with no obvious domain(s) or structural motif. Phosphorylation on Ser233, however, may regulate the overall interaction of the NUP107‑160 subcomplex with the much larger nuclear pore. There one potential splice form that shows an alternative start site at Met47. Full-length NUP85 shares 92% aa identity with mouse NUP85.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • Q9BW27(Human)