NTS2/NTSR2 Antibody

Product Discontinued

NTS2/NTSR2 Antibody Summary

• Immunogen: Synthetic peptide, corresponding to amino acids 151-200 of Human NTR2.
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NTSR2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry Free-Floating
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:1000
• Application Notes: Use in IHC-FrFl reported in scientific literature (PMID:34310753). Western blot (WB). analysis of NTR2 (D184). pAb in extracts from Jurkat cells.
• Theoretical MW: 45 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2)
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for NTS2/NTSR2 Antibody

• neurotensin receptor 2
• neurotensin receptor type 2
• neurotensin receptor, type 2
• NT-R-2
• NTR2Levocabastine-sensitive neurotensin receptor
• NTS2
• NTSR2

Background

Neurotensin (NT) initiates an intracellular response by interacting with the G protein-coupled receptors NTR1 (NTS1 receptor, high affinity NTR) and NTR2 (NTS2 receptor, levocabastine-sensitive neurotensin receptor), and the type I receptor NTR3 (NTS3 receptor, sortilin-1, Gp95). NT has a wide distribution in regions of the brain and in peripheral tissues where NT receptors can contribute to hypotension, hyperglycemia, hypothermia, antinociception and regulation of intestinal motility and secretion. HL-60 cells express NTR1, which can couple to Gq, Gi/o or Gs. Alternative splicing of rat NTR2 can generate a 5-transmembrane domain variant isoform that is coexpressed with the full length NTR2 throughout the brain and spinal cord. NTR3 activation in the murine microglial cell line N11 induces MIP-2, MCP-1, IL-1beta and TNFalpha in an ERK1/2 and Akt kinase-dependent manner.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Neurotensin Receptor 2 Antibody (NBP1-00959)(2)

Publications using NBP1-00959

• Grigorios K, Anne B, AngElique B et al. Neurotensin receptor 2 is induced in astrocytes and brain endothelial cells in relation to status epilepticus and neuroinflammation following pilocarpine administration in rats bioRxiv 2020-06-30
• Kyriatzis G, Bernard A, Bole A Et Al. Neurotensin receptor 2 is induced in astrocytes and brain endothelial cells in relation to neuroinflammation following pilocarpine-induced seizures in rats Glia 2021-07-26 [PMID: 34310753] (IHC-FrFl)

Bioinformatics

• Gene Symbol: NTSR2
• Entrez:
  • 23620(Human)
  • 64636(Rat)