NTH1 Antibody (208521) [Unconjugated]

Western blot shows lysates of DU145 human prostate carcinoma cell line and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Human NTH1 Monoclonal Antibody (Catalog # MAB2675) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF007). A specific band was detected for NTH1 at approximately 38 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu
• Applications: WB
• Clone: 208521
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

NTH1 Antibody (208521) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human NTH1
  Met1-Leu312
  Accession # P78549
• Specificity: Detects human NTH1 in Western blots.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: NTHL1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for NTH1 Antibody (208521) [Unconjugated]

• EC 4.2.99.18
• endonuclease III-like protein 1
• nth endonuclease III-like 1 (E. coli)
• NTH1
• NTHL1
• OCTS3
• OCTS3NTH1nth (E.coli endonuclease III)-like 1

Background

NTH1 is a DNA glycosylase with apurinic lyase activity. It functions in the repair of oxidative damage to DNA through the BER (base excision repair) pathway.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NTH1 Antibody (MAB2675)(4)

Publications using MAB2675

• Paul W Doetsch Identification of SUMO modification sites in the base excision repair protein, Ntg1 DNA Repair (Amst.), 2016-10-31;48(0):51-62. 2016-10-31 [PMID: 27839712] (Western Blot, Human)
• Crooks D, Natarajan T, Jeong S, Chen C, Park S, Huang H, Ghosh M, Tong W, Haller R, Wu C, Rouault T Elevated FGF21 secretion, PGC-1alpha and ketogenic enzyme expression are hallmarks of iron-sulfur cluster depletion in human skeletal muscle. Hum Mol Genet, 2013-08-13;23(1):24-39. 2013-08-13 [PMID: 23943793] (Western Blot, Human)
• Yanbo Shi, Manik Ghosh, Gennadiy Kovtunovych, Daniel R. Crooks, Tracey A. Rouault Both human ferredoxins 1 and 2 and ferredoxin reductase are important for iron-sulfur cluster biogenesis Biochimica et Biophysica Acta (BBA) - Molecular Cell Research 2012-02-01 [PMID: 22101253]
• Goto M, Shinmura K, Igarashi H, Kobayashi M, Konno H, Yamada H, Iwaizumi M, Kageyama S, Tsuneyoshi T, Tsugane S, Sugimura H Altered expression of the human base excision repair gene NTH1 in gastric cancer. Carcinogenesis, 2009-05-04;30(8):1345-52. 2009-05-04 [PMID: 19414504] (Western Blot, Human)

Bioinformatics

• Gene Symbol: NTHL1
• Uniprot:
  • P78549()