NT5C3 Antibody

Western Blot: NT5C3 Antibody [NBP3-00212] - Anti-NT5C3 rabbit polyclonal antibody at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate. Lane B: HepG2 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 38 kDa. Observed band size: 37 kDa (We are unsure as to the identity of these extra bands).

Western Blot: NT5C3 Antibody [NBP3-00212] - Anti-NT5C3 rabbit polyclonal antibody at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate. Lane B: HepG2 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 38 kDa. Observed band size: 37 kDa (We are unsure as to the identity of these extra bands).

Immunoprecipitation: NT5C3 Antibody [NBP3-00212] - NT5C3 was immunoprecipitated using: Lane A: 0.5 mg Jurkat Whole Cell Lysate. Lane B: 0.5 mg HepG2 Whole Cell Lysate 2 ul anti-NT5C3 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-NT5C3 rabbit polyclonal antibody, at 1:200 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 38 kDa. Observed band size: 38 kDa

Product Discontinued

NT5C3 Antibody Summary

• Description: This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
• Immunogen: Produced in rabbits immunized with purified, recombinant Human NT5C3 (Accession#: NP_001002010; Met12-Leu297)
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NT5C3A
• Purity: Antigen and protein A Affinity-purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:5000-1:10000
  • Immunoprecipitation 2-4 uL/mg of lysate
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.2 um filtered solution in PBS
• Preservative: No Preservative
• Purity: Antigen and protein A Affinity-purified

Alternate Names for NT5C3 Antibody

• 5'-nucleotidase, cytosolic III
• cN-IIIMGC87828
• cytosolic 5'-nucleotidase 3
• Cytosolic 5'-nucleotidase III
• p36
• P5N1
• P5N-1
• P5'N-1MGC27337
• PN-IMGC87109
• PSN1UMPH
• Pyrimidine 5'-nucleotidase 1
• UMPH1EC 3.1.3.5
• uridine 5' monophosphate hydrolase 1
• Uridine 5'-monophosphate hydrolase 1

Background

Plasmid: pCMV-NT5C3 Full length; Host Cell: 293T

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NT5C3A