NSF Antibody

Product Discontinued

NSF Antibody Summary

• Immunogen: Synthetic peptide corresponding to amino acids around 731 of rat NSF.
• Specificity: The antibody recognizes 85 kDa NSF of mouse, and rat origins.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NSF
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 10 - 20 ug/mL
  • Immunoprecipitation 5 - 15 ug/mL
  • Western Blot 1 - 2 ug/mL
• Theoretical MW: 83 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 1% BSA, 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Affinity purified

Alternate Names for NSF Antibody

• DEE96
• EC 3.6.4.6
• NEM-sensitive fusion protein
• N-ethylmaleimide-sensitive factor
• N-ethylmaleimide-sensitive factor-like protein
• N-ethylmaleimide-sensitive fusion protein
• NSF
• SEC18
• SKD2
• vesicle-fusing ATPase
• Vesicular-fusion protein NSF

Background

N ethylmaleimide sensitive factor (NSF) is an approx. 83 kDa protein (predicted MW) which was initially isolated based on its ability to restore intercisternal Golgi transport to N ethylmaleimide treated membranes. NSF was subsequently discovered to be involved in a variety of membrane fusion events. NSF is an ATPase with two ATP binding domains that are essential for membrane fusion and homo oligomerization. In order to interact with target membranes, NSF requires another set of soluble proteins called Soluble NSF attachment proteins (SNAPs). NSF has been shown to play a prominent role in synaptic vesicle fusion. Together with synaptotagmin and a SNAP, NSF modulates the interaction of SNAP25, VAMP, and syntaxin, in an ATP dependent manner, to form a complex with a sedimentation coefficient of 20 S. This complex may represent an intermediate involved in synaptic vesicle docking and fusion.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NSF
• Entrez:
  • 18195(Mouse)
  • 60355(Rat)
• Uniprot:
  • Q9QUL6(Rat)