NSD3 Antibody

Western Blot: NSD3 Antibody [NBP1-04991] - Detection of NSD3 (isoform 3) in HeLa nuclear extracts using NBP1-04991.

Product Discontinued

NSD3 Antibody Summary

• Immunogen: Synthetic peptide made to an internal portion of the human NSD3 protein (within residues 500-600). [Swiss-Prot# Q9BZ95]
• Localization: Nucleus
• Specificity: This has been observed to recognize a band that corresponds to isoform 3 of NSD3.
• Predicted Species: Bovine (90%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: WHSC1L1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/ml
• Application Notes: This NSD3 antibody is useful for Western blot, where a band is seen at ~70 kDa corresponding to isoform 3 of NSD3. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Theoretical MW: 70 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  HeLa Nuclear Cell Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.1% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for NSD3 Antibody

• EC 2.1.1.43
• FLJ20353
• histone-lysine N-methyltransferase NSD3
• MGC126766
• MGC142029
• NSD3DKFZp667H044
• Nuclear SET domain-containing protein 3
• pp14328
• Protein whistle
• WHSC1-like 1 isoform 9 with methyltransferase activity to lysine
• WHSC1-like protein 1
• Wolf-Hirschhorn syndrome candidate 1-like 1
• Wolf-Hirschhorn syndrome candidate 1-like protein 1

Background

NSD family of SET domain-containing histone methyltransferases includes NSD1, NSD2 (also known as WHSC1, Wolf-Hirschhorn syndrome candidate 1 or MMSET, multiple myeloma SET), and NSD3 (also known as WHSC1L1, WHSC1 like 1). All the three NSD proteins have been directly linked to multiple human diseases and each NSD family member behaves as an oncogene. HSD3 is a istone methyltransferase that preferentially methylates 'Lys-4' and 'Lys-27' of histone H3. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation, while 'Lys-27' is a mark for transcriptional repression. Translocations resulting in NUP98 fusion to NSD1 and NSD3 leads to the development of acute myeloid leukemia (AML) and overexpression of NSD3 via gene amplification occurs in several breast cancer cell lines as well as in non small cell lung carcinomas (NSCLC).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NSD3 Antibody (NBP1-04991)(1)

Publication using NBP1-04991

• Yang ZQ, Liu G, Bollig-Fischer A et al. Transforming properties of 8p11-12 amplified genes in human breast cancer Cancer Res 2010-11-01 [PMID: 20940404] (WB, Human)

Review for NSD3 Antibody (NBP1-04991) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: GANG LIU WB Human 12/08/2010

Summary

• Application: Western Blot
• Sample Tested: MCF10A/NSD3 whole cell lysate, Sample Amount: 40ug
• Species: Human
• Lot: A

Bioinformatics

• Gene Symbol: WHSC1L1
• Entrez:
  • 54904(Human)
• Uniprot:
  • Q9BZ95(Human)