NSD3 Antibody

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Western Blot: NSD3 Antibody [NBP1-04991] - Detection of NSD3 (isoform 3) in HeLa nuclear extracts using NBP1-04991.

Product Details

Summary
Reactivity Hu, BvSpecies Glossary
Applications WB, ICC/IF (-)
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

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Catalog# & Conjugate Size Price

NSD3 Antibody Summary

Immunogen
Synthetic peptide made to an internal portion of the human NSD3 protein (within residues 500-600). [Swiss-Prot# Q9BZ95]
Localization
Nucleus
Specificity
This has been observed to recognize a band that corresponds to isoform 3 of NSD3.
Predicted Species
Bovine (90%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
WHSC1L1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/ml
Application Notes
This NSD3 antibody is useful for Western blot, where a band is seen at ~70 kDa corresponding to isoform 3 of NSD3. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
70 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
NSD3 Lysate (NBL1-17849)
HeLa Lysate (NB800-PC9)
Reviewed Applications
Read 1 Review rated 4
using
NBP1-04991 in the following applications:

Publications
Read Publication using
NBP1-04991 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.1% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for NSD3 Antibody

  • EC 2.1.1.43
  • FLJ20353
  • histone-lysine N-methyltransferase NSD3
  • MGC126766
  • MGC142029
  • NSD3DKFZp667H044
  • Nuclear SET domain-containing protein 3
  • pp14328
  • Protein whistle
  • WHSC1-like 1 isoform 9 with methyltransferase activity to lysine
  • WHSC1-like protein 1
  • Wolf-Hirschhorn syndrome candidate 1-like 1
  • Wolf-Hirschhorn syndrome candidate 1-like protein 1

Background

NSD family of SET domain-containing histone methyltransferases includes NSD1, NSD2 (also known as WHSC1, Wolf-Hirschhorn syndrome candidate 1 or MMSET, multiple myeloma SET), and NSD3 (also known as WHSC1L1, WHSC1 like 1). All the three NSD proteins have been directly linked to multiple human diseases and each NSD family member behaves as an oncogene. HSD3 is a istone methyltransferase that preferentially methylates 'Lys-4' and 'Lys-27' of histone H3. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation, while 'Lys-27' is a mark for transcriptional repression. Translocations resulting in NUP98 fusion to NSD1 and NSD3 leads to the development of acute myeloid leukemia (AML) and overexpression of NSD3 via gene amplification occurs in several breast cancer cell lines as well as in non small cell lung carcinomas (NSCLC).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for NSD3 Antibody (NBP1-04991)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


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WB
(1)
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Review for NSD3 Antibody (NBP1-04991) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP1-04991:
Filter by Applications
WB
(1)
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Filter by Species
Human
(1)
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Images Ratings Applications Species Date Details
 
reviewed by:
GANG LIU
WB Human 12/08/2010
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Summary

ApplicationWestern Blot
Sample TestedMCF10A/NSD3 whole cell lysate, Sample Amount: 40ug
SpeciesHuman
LotA
CommentsWe tested the NSD3/WHSC1L1 Ab(NBP1-04991)in Human mammary epithelial cell line MCF10A model which over-expression NSD3/WHSC1L1 short isoform (NM_017778.2) by Invitrogen lentiviral system (High-Perform pLenti-DEST).
The stable MCF10A/NSD3-short clone expressing high level of NSD3-short protein could be detected by Ab(NBP1-04991). Please see Supplementary figures(Figure S1-B)for reference [Cancer Res. 2010 Nov 1;70(21):8487-97.]
Pub Med ID20940404

Blocking

Blocking DetailsBlocking Buffer: 5% BSA in TBST, Blocking Time: 1 hour, Blocking Temp: Room temperature

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:1000, Incubation Dilution Buffer: 5% BSA in TBST, Incubation Time: overnight, Incubation Temp: 4C

Secondary Antibody

Secondary DescriptionSecondary Ab: Anti-Rabbit IgG (PEROXIDASE LABELED), Secondary Ab Dilution Ratio: 1:2500
Secondary Manufacturer Cat#Vector Laboratories (PI-1000) Lot: s0327

Details

Detection NotesDetection Method: Film, Exposure Time: 3min, Pos Ctrl: MCF10A/NSD3, Neg Ctrl: MCF10A, Specific Bands: 70 kDa

Comments

CommentsWe tested the NSD3/WHSC1L1 Ab(NBP1-04991)in Human mammary epithelial cell line MCF10A model which over-expression NSD3/WHSC1L1 short isoform (NM_017778.2) by Invitrogen lentiviral system (High-Perform pLenti-DEST).
The stable MCF10A/NSD3-short clone expressing high level of NSD3-short protein could be detected by Ab(NBP1-04991). Please see Supplementary figures(Figure S1-B)for reference [Cancer Res. 2010 Nov 1;70(21):8487-97.]

Product General Protocols

View specific protocols for NSD3 Antibody (NBP1-04991): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for NSD3 Antibody (NBP1-04991) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

DyLight 405 Labeled NBP1-04991V
FITC Labeled NBP1-04991F

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GANG LIU
12/08/2010
Application: WB
Species: Human

Bioinformatics

Gene Symbol WHSC1L1
Entrez
Uniprot