NSD3 Antibody (7B4) - BSA Free

Western Blot: NSD3 Antibody (7B4) [NBP1-04324] - The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NSD3 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: 293T cell lysate Lane 2.: HeLa cell lysate Lane 3.: MCF7 cell lysate Lane 4.: NIH3T3 cell lysate Lane 5.: U87MG cell lysate

• Reactivity: Hu
• Applications: WB, ELISA
• Clone: 7B4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

NSD3 Antibody (7B4) - BSA Free Summary

• Description: Novus Biologicals Mouse NSD3 Antibody (7B4) - BSA Free (NBP1-04324) is a monoclonal antibody validated for use in WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant human NSD3 (383-660aa) purified from E. coli
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: NSD3
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Western Blot 1:1000-1:3000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4), 10% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for NSD3 Antibody (7B4) - BSA Free

• EC 2.1.1.43
• FLJ20353
• histone-lysine N-methyltransferase NSD3
• MGC126766
• MGC142029
• NSD3DKFZp667H044
• Nuclear SET domain-containing protein 3
• pp14328
• Protein whistle
• WHSC1-like 1 isoform 9 with methyltransferase activity to lysine
• WHSC1-like protein 1
• Wolf-Hirschhorn syndrome candidate 1-like 1
• Wolf-Hirschhorn syndrome candidate 1-like protein 1

Background

This gene encoding NSD3 is related to the Wolf-Hirschhorn syndrome candidate - 1 gene and encodes a protein with PWWP (proline-tryptophan-tryptophan-proline) domains. NSD3 is Histone methyltransferase which preferentially methylates 'Lys-4' and 'Lys-27' of histone H3 and is amplified in several tumor cell lines and primary breast carcinomas.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NSD3
• Uniprot:
  • NP_075447(Human)