Recombinant Human NRIF3 His Protein

SDS-Page: NRIF3 Protein [NBP2-23143]

• Reactivity: Hu
• Applications: PAGE
• Concentration: 0.5 mg/ml

Recombinant Human NRIF3 His Protein Summary

• Description: A denatured recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-216 of Human NRIF3
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMPFAPVA QARVQWHDFR SLQHLLPAFK RFSCLSLGSS WDYSVKRSLK LDGLLEENSF DPSKITRKKS VITYSPTTGT CQMSLFASPT SSEEQKHRNG LSNEKRKKLN HPSLTESKES TTKDNDEFMM LLSKVEKLSE EIMEIMQNLS SIQALEGSRE LENLIGISCA SHFLKREMQK TKELMTKVNK QKLFEKSTGL PHKASRHLDS YEFLKAILN

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: ITGB3BP
• Purity: >90%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Application Notes: Denatured protein is most likely not the best option for functional studies. It is better suited for Western Blot (WB) or imaging assays.
• Theoretical MW: 27.1 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH 8.0), 0.4M UREA, 10% glycerol
• Preservative: No Preservative
• Concentration: 0.5 mg/ml
• Purity: >90%, by SDS-PAGE

Alternate Names for Recombinant Human NRIF3 His Protein

• beta-3-endonexin
• CENP-R
• centromere protein R
• HSU37139
• integrin beta 3 binding protein (beta3-endonexin)
• Integrin beta-3-binding protein
• NRIF3CENPRbeta 3 endonexin
• Nuclear receptor-interacting factor 3
• TAP20

Background

Cntromere protein R isoform 1, also known as ITGB3BP1, is a widely expressed protein that contains a DD1 or RepD1 domain and an LXXLL motif. Induced by estrogen, ITGB3BP1 is believed to function as a nuclear receptor coactivator. It also associates with the CENP-A-CAD complex which is involved in mitotic progression, the assembly of kinetochore proteins and chromosome segregation. It acts as a transcriptional corepressor via its interaction with the NFKB1 NF-kappa-B subunit, possibly by interfering with the transactivation domain of NFKB1. Recombinant human ITGB3BP1 protein, fused to His-tag at N-terminus, was expressed in E.coli.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: ITGB3BP