Mouse Nrf1 ELISA Kit (Colorimetric)

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ELISA: Mouse Nrf1 ELISA Kit (Colorimetric) [NBP2-76757] - Samples were spiked with high concentrations of Mouse Nrf1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range ...read more
ELISA: Mouse Nrf1 ELISA Kit (Colorimetric) [NBP2-76757] - Standard Curve Reference

Product Details

Summary
Reactivity MuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and other biological fluids
Standard Curve Range
0.156 - 10ng/mL
Sensitivity
0.094ng/mL

Order Details

Mouse Nrf1 ELISA Kit (Colorimetric) Summary

Standard Curve Range
0.156 - 10ng/mL
Sensitivity
0.094ng/mL
Assay Type
Sandwich-ELISA
Inter-Assay
CV% < 5.37
Intra-Assay
CV% < 5.5
Spike Recovery
83-106%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
NRF1

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro ELISA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Stop Solution
  12. Substrate Reagent

Alternate Names for Mouse Nrf1 ELISA Kit (Colorimetric)

  • Alpha palindromic-binding protein
  • ALPHA-PAL
  • EWG
  • HBZ17
  • LCR-F1
  • NFE2L1
  • Nrf1
  • NRF-1
  • nuclear respiratory factor 1
  • TCF11

Background

NRF1 encodes a protein that homodimerizes and functions as a transcription factor which activates the expression of some key metabolic genes regulating cellular growth and nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication. The protein has also been associated with the regulation of neurite outgrowth. Alternate transcriptional splice variants, which encode the same protein, have been characterized. Additional variants encoding different protein isoforms have been described but they have not been fully characterized. Confusion has occurred in bibliographic databases due to the shared symbol of NRF1 for this gene and for "nuclear factor (erythroid-derived 2)-like 1" which has an official symbol of NFE2L1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Product General Protocols

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PGC-1 alpha: Roles in Mitochondrial Biogenesis and Disease
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Exploring the Many Roles of PGC-1 alpha
The peroxisome proliferator-activated receptor gamma, co-activator 1 (PGC-1 alpha or PPARGC1A) gene encodes a 91 kDa nuclear protein that acts as a transcriptional co-activator involved in energy metabolism. Interaction with PPAR gamma allows it to in...  Read full blog post.

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Bioinformatics

Gene Symbol NRF1