Nrf1 Antibody (JM89-63)

Western Blot: Nrf1 Antibody (JM89-63) [NBP2-75597] - Western blot analysis of Nrf1 on Hela cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry/Immunofluorescence: Nrf1 Antibody (JM89-63) [NBP2-75597] - Staining NRF1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: Nrf1 Antibody (JM89-63) [NBP2-75597] - Analysis of paraffin-embedded rat brain tissue using anti-NRF1 antibody. Counter stained with hematoxylin.

Flow Cytometry: Nrf1 Antibody (JM89-63) [NBP2-75597] - Analysis of 293T cells with NRF1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).

Western Blot: Nrf1 Antibody (JM89-63) [NBP2-75597] - Analysis of NRF1 on Hela cell (1) and mouse heart tissue (2) lysate using anti-NRF1 antibody at 1/500 dilution.

Immunocytochemistry/Immunofluorescence: Nrf1 Antibody (JM89-63) [NBP2-75597] - Staining NRF1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: Nrf1 Antibody (JM89-63) [NBP2-75597] - Staining NRF1 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: Nrf1 Antibody (JM89-63) [NBP2-75597] - Analysis of paraffin-embedded human tonsil tissue using anti-NRF1 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Nrf1 Antibody (JM89-63) [NBP2-75597] - Analysis of paraffin-embedded human thyriod tissue using anti-NRF1 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Nrf1 Antibody (JM89-63) [NBP2-75597] - Analysis of paraffin-embedded mouse colon tissue using anti-NRF1 antibody. Counter stained with hematoxylin.

Product Discontinued

Nrf1 Antibody (JM89-63) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human Nrf1 aa 333-382 / 503. (SwissProt: Q16656 Human; SwissProt: Q9WU00 Mouse; SwissProt: Q62792 Rat)
• Localization: Nucleus.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: NRF1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation
  • Western Blot 1:500-1:2000
• Theoretical MW: 56 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Nrf1 Antibody (JM89-63)

• Alpha palindromic-binding protein
• ALPHA-PAL
• EWG
• HBZ17
• LCR-F1
• NFE2L1
• Nrf1
• NRF-1
• nuclear respiratory factor 1
• TCF11

Background

NRF1 encodes a protein that homodimerizes and functions as a transcription factor which activates the expression of some key metabolic genes regulating cellular growth and nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication. The protein has also been associated with the regulation of neurite outgrowth. Alternate transcriptional splice variants, which encode the same protein, have been characterized. Additional variants encoding different protein isoforms have been described but they have not been fully characterized. Confusion has occurred in bibliographic databases due to the shared symbol of NRF1 for this gene and for "nuclear factor (erythroid-derived 2)-like 1" which has an official symbol of NFE2L1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NRF1