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Nrf1 Antibody (JM89-63)

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Western Blot: Nrf1 Antibody (JM89-63) [NBP2-75597] - Analysis of NRF1 on Hela cell (1) and mouse heart tissue (2) lysate using anti-NRF1 antibody at 1/500 dilution.
Western Blot: Nrf1 Antibody (JM89-63) [NBP2-75597] - Analysis of NRF1 on Hela cell (1) and mouse heart tissue (2) lysate using anti-NRF1 antibody at 1/500 dilution.
Immunocytochemistry/ Immunofluorescence: Nrf1 Antibody (JM89-63) [NBP2-75597] - Staining NRF1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with ...read more
Immunohistochemistry-Paraffin: Nrf1 Antibody (JM89-63) [NBP2-75597] - Analysis of paraffin-embedded rat brain tissue using anti-NRF1 antibody. Counter stained with hematoxylin.
Flow Cytometry: Nrf1 Antibody (JM89-63) [NBP2-75597] - Analysis of 293T cells with NRF1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
Immunocytochemistry/ Immunofluorescence: Nrf1 Antibody (JM89-63) [NBP2-75597] - Staining NRF1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with ...read more
Immunocytochemistry/ Immunofluorescence: Nrf1 Antibody (JM89-63) [NBP2-75597] - Staining NRF1 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with ...read more
Immunohistochemistry-Paraffin: Nrf1 Antibody (JM89-63) [NBP2-75597] - Analysis of paraffin-embedded human tonsil tissue using anti-NRF1 antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: Nrf1 Antibody (JM89-63) [NBP2-75597] - Analysis of paraffin-embedded human thyriod tissue using anti-NRF1 antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: Nrf1 Antibody (JM89-63) [NBP2-75597] - Analysis of paraffin-embedded mouse colon tissue using anti-NRF1 antibody. Counter stained with hematoxylin.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P, IP
Clone
JM89-63
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

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Nrf1 Antibody (JM89-63) Summary

Additional Information
Recombinant Monoclonal Antibody.
Immunogen
Recombinant Protein
Localization
Nucleus.
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
NRF1
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation
Theoretical MW
54 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
TBS (pH7.4), 1%BSA, 40%Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for Nrf1 Antibody (JM89-63)

  • Alpha palindromic-binding protein
  • ALPHA-PAL
  • EWG
  • HBZ17
  • LCR-F1
  • NFE2L1
  • Nrf1
  • NRF-1
  • nuclear respiratory factor 1
  • TCF11

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Immunocytochemistry/Immunofluorescence: mtTFA Antibody (18G102B2E11) [NBP1-71648] - Immunostaining in HeLa cells. C1: DAPI staining 2 nuclei, C2: anti-mtTFA stain with Alexa Fluor 488 secondary ab (green), C3: Mitotracker Red staining mitochondria. Image courtesy of Elizabeth Wang.
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Western Blot: PGC1 alpha Antibody [NBP1-04676] - Total protein from human adipose and skeletal muscle tissue, HeLa and A431 cells lines was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/mL PGC1 alpha Antibody (Molecular weight: 91 KDa) in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence
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Publications for Nrf1 Antibody (NBP2-75597) (0)

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Bioinformatics

Gene Symbol NRF1