Recombinant Human NQO-1 Protein

Product Discontinued

Recombinant Human NQO-1 Protein Summary

• Description: A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-274 of Human NQO-1
  
  

  Source: E.coli

  Amino Acid Sequence: Sequences of amino acids: MGSSHHHHHH SSGLVPRGSH MVGRRALIVL AHSERTSFNY AMKEAAAAAL KKKGWEVVES DLYAMNFNPI ISRKDITGKL KDPANFQYPA ESVLAYKEGH LSPDIVAEQK KLEAADLVIF QFPLQWFGVP AILKGWFERV FIGEFAYTYA AMYDKGPFRS KKAVLSITTG GSGSMYSLQG IHGDMNVILW PIQSGILHFC GFQVLEPQLT YSIGHTPADA RIQILEGWKK RLENIWDETP LYFAPSSLFD LNFQAGFLMK KEVQDEEKNK KFGLSVGHHL GKSIPTDNQI KARK

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: NQO1
• Purity: >95% pure by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 33 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH 8.0), 10% glycerol, 1 mM DTT
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: >95% pure by SDS-PAGE

Alternate Names for Recombinant Human NQO-1 Protein

• Azoreductase
• DHQU
• DIA4
• DIA4azoreductase
• diaphorase (NADH/NADPH) (cytochrome b-5 reductase)
• Diaphorase-4
• Dioxin-inducible 1
• DTD
• DTDEC 1.6.5.2
• DT-diaphorase
• Menadione Reductase
• NAD(P)H dehydrogenase [quinone] 1
• NAD(P)H dehydrogenase, quinone 1
• NAD(P)H:menadione oxidoreductase 1
• NAD(P)H:Quinone acceptor oxidoreductase type 1
• NAD(P)H:quinone oxidoreductase 1
• NAD(P)H:quinone oxireductase
• NMOR1
• NMOR1diaphorase-4
• NQO1
• NQO-1
• Phylloquinone reductase
• QR1
• QR1NMORI
• Quinone Reductase 1

Background

NQO1 is a member of the NAD(P)H dehydrogenase (quinone) family and encodes a cytoplasmic 2-electron reductase. This protein apparently serves as a quinone reductase in connection with conjugation reactions of hydroquinons involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis. NQO1 functions as an important part of cellular antioxidant defense by detoxifying quinines thus preventing the formation of reactive oxygen species. Altered expression of NQO1 has been seen in many tumors and is also associated with Alzheimer's disease (AD). Recombinant NQO1 protein was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: NQO1