NQO-1 Antibody

Western Blot: NQO-1 Antibody [NBP2-23792] - Analysis of NQO1 in human spleen lysate using NBP2-23792 at A) 1:1000 and B) 1:500.

Immunohistochemistry-Paraffin: NQO-1 Antibody [NBP2-23792] - Analysis of NQO1 in formalin-fixed, paraffin-embedded human stomach tumor using an isotype control (top left) and NBP2-23792(bottom left, right) at 7 ug/ml.

Product Discontinued

NQO-1 Antibody Summary

• Immunogen: A portion of amino acid 250-274 of human NQO1 was used as the immunogen.
• Predicted Species: Mouse (90%), Chimpanzee (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NQO1
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 7 ug/ml
  • Western Blot 1:500-1: 1000

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: 100% homologous in human, monkey, chimpanzee and dog; rat (95%), mouse (90%) and chicken (83%).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 0.1 ml crude whole Rabbit sera with 50% Glycerol
• Preservative: 0.025% Sodium Azide
• Purity: Unpurified

Alternate Names for NQO-1 Antibody

• Azoreductase
• DHQU
• DIA4
• DIA4azoreductase
• diaphorase (NADH/NADPH) (cytochrome b-5 reductase)
• Diaphorase-4
• Dioxin-inducible 1
• DTD
• DTDEC 1.6.5.2
• DT-diaphorase
• Menadione Reductase
• NAD(P)H dehydrogenase [quinone] 1
• NAD(P)H dehydrogenase, quinone 1
• NAD(P)H:menadione oxidoreductase 1
• NAD(P)H:Quinone acceptor oxidoreductase type 1
• NAD(P)H:quinone oxidoreductase 1
• NAD(P)H:quinone oxireductase
• NMOR1
• NMOR1diaphorase-4
• NQO1
• NQO-1
• Phylloquinone reductase
• QR1
• QR1NMORI
• Quinone Reductase 1

Background

NQO1 is a 274 amino acid protein belonging to the NAD(P)H dehydrogenase (quinone) family. Its enzymatic activity prevents reduction of quinones that results in the production of radical species and is involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis. It plays a role in regulating ubiquitin-independent degradation of ornithine decarboxylase by the 20S proteasome and regulates p53 proteasomal degradation in cancer. Defects in NQO1 lead to tardive dyskinesia (TD), an increased risk of hematotoxicity after exposure to benzene, susceptibility to various forms of cancer and Alzheimer's disease (AD). It is widely expressed.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NQO1
• Uniprot:
  • P15559(Human)