NQO-1 Antibody

Images

 
Western Blot: NQO-1 Antibody [NB100-1005] - Staining of Rat (A) and Pig (B) Kidney lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence: NQO-1 Antibody [NB100-1005] - analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (5ug/ml) followed by Alexa Fluor 488 ...read more
Immunohistochemistry-Paraffin: NQO-1 Antibody [NB100-1005] - Staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Western Blot: NQO-1 Antibody [NB100-1005] - Staining of Human Kidney lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Product Details

Summary
Reactivity Hu, Rt, PoSpecies Glossary
Applications WB, ICC/IF, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

NQO-1 Antibody Summary

Immunogen
Peptide with sequence C-SIPTDNQIKARK corresponding to C-Terminus according to NP_000894.1, NP_001020604.1, NP_001020605.1.
Specificity
This products is expected to recognize all three reported isoforms (NP_000894.1, NP_001020604.1 and NP_001020605.1).
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
NQO1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.03 ug/ml
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Peptide ELISA Detection limit 1:64000
Application Notes
WB: Approx. 30 kDa band observed in human kidney and lung lysates and in rat and porcine kidney lysates (calculated MW of 30.9 kDa band according to NP_000894.1). IHC: Paraffin embedded Human Kidney.
Positive Control
NQO-1 Lysate (NBL1-13761)
Publications
Read Publications using
NB100-1005 in the following applications:

  • WB
    1 publication

Reactivity Notes

Expected from sequence similarity: Canine

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for NQO-1 Antibody

  • Azoreductase
  • DHQU
  • DIA4
  • DIA4azoreductase
  • diaphorase (NADH/NADPH) (cytochrome b-5 reductase)
  • Diaphorase-4
  • Dioxin-inducible 1
  • DTD
  • DTDEC 1.6.5.2
  • DT-diaphorase
  • Menadione Reductase
  • NAD(P)H dehydrogenase [quinone] 1
  • NAD(P)H dehydrogenase, quinone 1
  • NAD(P)H:menadione oxidoreductase 1
  • NAD(P)H:Quinone acceptor oxidoreductase type 1
  • NAD(P)H:quinone oxidoreductase 1
  • NAD(P)H:quinone oxireductase
  • NMOR1
  • NMOR1diaphorase-4
  • NQO1
  • NQO-1
  • Phylloquinone reductase
  • QR1
  • QR1NMORI
  • Quinone Reductase 1

Background

NQO1 is a 2-electron reductase that detoxifies quinones derived from the oxidation of phenolic metabolites of benzene. Individuals homozygous for the T/T form of the 609C-T polymorphism have an increased risk of benzene hematotoxicity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for NQO-1 Antibody (NB100-1005)(3)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for NQO-1 Antibody (NB100-1005) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol NQO1
Entrez
Uniprot