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NQO-1 Antibody (JF440-1)

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Western Blot: NQO-1 Antibody (JF440-1) [NBP2-66990] - Western blot analysis of NQO-1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: mouse kidney tissue lysate Lane 2: SH-SY5Y cell lysate
Western Blot: NQO-1 Antibody (JF440-1) [NBP2-66990] - Western blot analysis of NQO-1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The ...read more
Flow Cytometry: NQO-1 Antibody (JF440-1) [NBP2-66990] - Analysis of Hela cells with NQO1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa ...read more
Western Blot: NQO-1 Antibody (JF440-1) [NBP2-66990] - Analysis of NQO1 on different lysates using anti-NQO1 antibody at 1/1,000 dilution. Positive control: Lane 1: SH-SY-5Y Lane 2: Mouse kidney Lane 3: Raji

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, ICC/IF, IP
Clone
JF440-1
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

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NQO-1 Antibody (JF440-1) Summary

Additional Information
Recombinant Monoclonal Antibody.
Immunogen
Synthetic peptide within N-terminal human NQO-1. (SwissProt: P15559 Human;SwissProt: Q64669 Mouse;SwissProt: P05982 Rat)
Localization
Cytoplasm.
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
NQO1
Purity
Protein A purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
  • Immunoprecipitation
  • Western Blot 1:1000-1:5000

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
TBS (pH7.4), 1%BSA, 40%Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for NQO-1 Antibody (JF440-1)

  • Azoreductase
  • DHQU
  • DIA4
  • DIA4azoreductase
  • diaphorase (NADH/NADPH) (cytochrome b-5 reductase)
  • Diaphorase-4
  • Dioxin-inducible 1
  • DTD
  • DTDEC 1.6.5.2
  • DT-diaphorase
  • Menadione Reductase
  • NAD(P)H dehydrogenase [quinone] 1
  • NAD(P)H dehydrogenase, quinone 1
  • NAD(P)H:menadione oxidoreductase 1
  • NAD(P)H:Quinone acceptor oxidoreductase type 1
  • NAD(P)H:quinone oxidoreductase 1
  • NAD(P)H:quinone oxireductase
  • NMOR1
  • NMOR1diaphorase-4
  • NQO1
  • NQO-1
  • Phylloquinone reductase
  • QR1
  • QR1NMORI
  • Quinone Reductase 1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Western Blot: Nrf2 Antibody [NBP1-32822] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NRF2 antibody [N2C2], Internal diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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Western Blot: CRYZ Antibody [NBP2-16017] - Sample (30 ug of whole cell lysate) A: Jurkat B: Raji 10% SDS PAGE gel, diluted at 1:1000.
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Western Blot: SOD1/Cu-Zn SOD Antibody [NBP2-24915] - Bovine ocular fluid. Antibody at 1:1000. Western blot image submitted by a verified customer review.
Immunohistochemistry-Paraffin: SOD1/Cu-Zn SOD Antibody [NBP2-24915] - Analysis of Superoxide Dismutase 1 in FFPE human liver tissue using an isotype control (top) and NBP2-24915 (bottom) at 5 ug/ml.
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(333116) [Unconjugate
MAB3024
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Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for Keap1 at approximately 75 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human/Mouse/Rat Keap1 Monoclonal Antibody (Catalog # MAB3024). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.
Western blot shows lysates of Jurkat human acute T cell leukemia parental cell line and Keap1 Jurkat cell line (KO). PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat Keap1 Monoclonal Antibody (Catalog # MAB3024) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Keap1 at approximately 66 kDa (as indicated) in the parental Jurkat cell line, but is not detectable in knockoutJurkat cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Western Blot: NQO-2 Antibody [NBP1-31563] - Sample(30 ug whole cell lysate)A:293T B:A431 12% SDS PAGE, antibody diluted at 1:1000.
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(2 Publications)
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Bioinformatics

Gene Symbol NQO1