NQO-1 Antibody (AI80)

Product Discontinued

NQO-1 Antibody (AI80) Summary

• Immunogen: Full length purified recombinant human NQO1
• Specificity: Human & Rat NQ01. Does not react with mouse NQO1
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: NQO1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Western Blot

Reactivity Notes

Reacts with Human and Rat.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS and 30% Glycerol
• Preservative: 0.05% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for NQO-1 Antibody (AI80)

• Azoreductase
• DHQU
• DIA4
• DIA4azoreductase
• diaphorase (NADH/NADPH) (cytochrome b-5 reductase)
• Diaphorase-4
• Dioxin-inducible 1
• DTD
• DTDEC 1.6.5.2
• DT-diaphorase
• Menadione Reductase
• NAD(P)H dehydrogenase [quinone] 1
• NAD(P)H dehydrogenase, quinone 1
• NAD(P)H:menadione oxidoreductase 1
• NAD(P)H:Quinone acceptor oxidoreductase type 1
• NAD(P)H:quinone oxidoreductase 1
• NAD(P)H:quinone oxireductase
• NMOR1
• NMOR1diaphorase-4
• NQO1
• NQO-1
• Phylloquinone reductase
• QR1
• QR1NMORI
• Quinone Reductase 1

Background

NQO1 is a member of the NAD(P)H dehydrogenase (quinone) family and encodes a cytoplasmic 2-electron reductase. This FAD-binding protein forms homodimers and reduces quinones to hydroquinones. NQO1's enzymatic activity prevents the one electron reduction of quinones that results in the production of radical species. NQO1 functions as an important part of cellular antioxidant defense by detoxifying quinones thus preventing the formation of reactive oxygen species. Mutations in the gene for NQO1 have been associated with tardive dyskinesia (TD), an increased risk of hematotoxicity after exposure to benzene, and susceptibility to various forms of cancer. Altered expression of NQO1 has been seen in many tumors and is also associated with Alzheimer's disease (AD). NQO1 levels have been shown to be raised in may tumor types. This has led to a search for anti-tumor agents that can be activated by NQO1 resulting in specific targeting of these compounds to the tumor site. Many cytotoxic drugs contain the quinone moiety, such as the benzoquinone group, which can be activated by NQO1 through the reduction into hydroquinone [which in this case can auto-oxidize and lead to the formation of free radicals and alkylating species]. However, the rat enzyme has been shown to be significantly more effective in activating some of these compounds than the human and mouse enzymes. This indicates that the available cytotoxic drugs are better substrates for the rat enzyme and are not the most ideal prodrugs for activation by NQO1 in human tumors.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NQO1
• Entrez:
  • 1728(Human)
  • 24314(Rat)