NQO-1 Antibody (4D12)

Western Blot: NQO-1 Antibody (4D12) [NBP2-37541] - Western blot analysis using NQO1 mouse mAb against A549 (1), SKNES (2), HepG2 (3), MCF-7 (4) and Hela (5) cell lysate.

Immunohistochemistry: NQO-1 Antibody (4D12) [NBP2-37541] - Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using NQO1 mouse mAb with DAB staining.

Flow Cytometry: NQO-1 Antibody (4D12) [NBP2-37541] - Flow cytometric analysis of NIH/3T3 cells using NQO1 mouse mAb (green) and negative control (red).

Western Blot: NQO-1 Antibody (4D12) [NBP2-37541] - Western blot analysis using NQO1 mAb against human NQO1 (AA: 134-274) recombinant protein. (Expected MW is 41.3 kDa)

Immunohistochemistry: NQO-1 Antibody (4D12) [NBP2-37541] - Immunohistochemical analysis of paraffin-embedded testis tissues using NQO1 mouse mAb with DAB staining.

ELISA: NQO-1 Antibody (4D12) [NBP2-37541] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Product Discontinued

NQO-1 Antibody (4D12) Summary

• Immunogen: Purified recombinant fragment of human NQO1 expressed in E. Coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: NQO1
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Flow Cytometry 1:200 - 1:400
  • Immunohistochemistry 1:200 - 1:1000
  • Immunohistochemistry-Paraffin 1:200 - 1:1000
  • Western Blot 1:500 - 1:2000
• Theoretical MW: 31 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 0.03% Sodium Azide
• Purity: Unpurified

Alternate Names for NQO-1 Antibody (4D12)

• Azoreductase
• DHQU
• DIA4
• DIA4azoreductase
• diaphorase (NADH/NADPH) (cytochrome b-5 reductase)
• Diaphorase-4
• Dioxin-inducible 1
• DTD
• DTDEC 1.6.5.2
• DT-diaphorase
• Menadione Reductase
• NAD(P)H dehydrogenase [quinone] 1
• NAD(P)H dehydrogenase, quinone 1
• NAD(P)H:menadione oxidoreductase 1
• NAD(P)H:Quinone acceptor oxidoreductase type 1
• NAD(P)H:quinone oxidoreductase 1
• NAD(P)H:quinone oxireductase
• NMOR1
• NMOR1diaphorase-4
• NQO1
• NQO-1
• Phylloquinone reductase
• QR1
• QR1NMORI
• Quinone Reductase 1

Background

NQO1 (NAD(P)H dehydrogenase [quinone] 1) is a 2-electron reductase that detoxifies quinones derived from the oxidation of phenolic metabolites of benzene. Individuals homozygous for the T/T form of the 609C-T polymorphism have an increased risk of benzene hematotoxicity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for NQO-1 Antibody (NBP2-37541). (Showing 1 - 2 of 2 FAQ).

1. Is it possible to get the amino acid sequence of the recombinant fragment used as the immunogen?
   • The immunogen used for this antibody was 134-274aa.
2. Is it possible to get the amino acid sequence of the recombinant NQO1 fragment used to generate this ab (4D12) cat # NBP2-37541?
   • Purified recombinant fragment of human NQO1 expressed in E. Coli. The immunogen consists of amino acids: 134-274.

Bioinformatics

• Gene Symbol: NQO1