NQO-1 Antibody (1E3-A6)

Images

 
Western Blot: NQO-1 Antibody (1E3-A6) [H00001728-M01] - NQO1 monoclonal antibody (M01), clone 1E3-A6 Analysis of NQO1 expression in HepG2.
Immunocytochemistry/ Immunofluorescence: NQO-1 Antibody (1E3-A6) [H00001728-M01] - Analysis of monoclonal antibody to NQO1 on HepG2 cell. Antibody concentration 10 ug/ml.
Western Blot: NQO-1 Antibody (1E3-A6) [H00001728-M01] - Analysis of NQO1 over-expressed 293 cell line, cotransfected with NQO1 Validated Chimera RNAi ( Cat # H00001728-R01V ) (Lane 2) or non-transfected control (Lane ...read more
Western Blot: NQO-1 Antibody (1E3-A6) [H00001728-M01] - Analysis of NQO1 expression in transfected 293T cell line by NQO1 monoclonal antibody (M01), clone 1E3-A6.Lane 1: NQO1 transfected lysate(30.9 KDa).Lane 2: ...read more
Immunoprecipitation: NQO-1 Antibody (1E3-A6) [H00001728-M01] - Analysis of NQO1 transfected lysate using anti-NQO1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with NQO1 MaxPab rabbit polyclonal ...read more
Sandwich ELISA: NQO-1 Antibody (1E3-A6) [H00001728-M01] - Detection limit for recombinant GST tagged NQO1 is approximately 0.3ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF, IP, RNAi
Clone
1E3-A6
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

NQO-1 Antibody (1E3-A6) Summary

Immunogen
NQO1 (AAH07659, 1 a.a. - 274 a.a.) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MVGRRALIVLAHSERTSFNYAMKEAAAAALKKKGWEVVESDLYAMNFNPIISRKDITGKLKDPANFQYPAESVLAYKEGHLSPDIVAEQKKLEAADLVIFQFPLQWFGVPAILKGWFERVFIGEFAYTYAAMYDKGPFRSKKAVLSITTGGSGSMYSLQGIHGDMNVILWPIQSGILHFCGFQVLEPQLTYSIGHTPADARIQILEGWKKRLENIWDETPLYFAPSSLFDLNFQAGFLMKKEVQDEEKNKKFGLSVGHHLGKSIPTDNQIKARK
Specificity
NQO1 - NAD(P)H dehydrogenase, quinone 1
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
NQO1
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • ELISA
  • Immunocytochemistry/Immunofluorescence
  • Immunoprecipitation
  • RNA Inhibition
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF, RNAi Validation and ELISA.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Purity
IgG purified

Notes

Quality control test: Antibody Reactive Against Recombinant Protein.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for NQO-1 Antibody (1E3-A6)

  • Azoreductase
  • DHQU
  • DIA4
  • DIA4azoreductase
  • diaphorase (NADH/NADPH) (cytochrome b-5 reductase)
  • Diaphorase-4
  • Dioxin-inducible 1
  • DTD
  • DTDEC 1.6.5.2
  • DT-diaphorase
  • Menadione Reductase
  • NAD(P)H dehydrogenase [quinone] 1
  • NAD(P)H dehydrogenase, quinone 1
  • NAD(P)H:menadione oxidoreductase 1
  • NAD(P)H:Quinone acceptor oxidoreductase type 1
  • NAD(P)H:quinone oxidoreductase 1
  • NAD(P)H:quinone oxireductase
  • NMOR1
  • NMOR1diaphorase-4
  • NQO1
  • NQO-1
  • Phylloquinone reductase
  • QR1
  • QR1NMORI
  • Quinone Reductase 1

Background

This gene is a member of the NAD(P)H dehydrogenase (quinone) family and encodes a cytoplasmic 2-electron reductase. This FAD-binding protein forms homodimers and reduces quinones to hydroquinones. This protein's enzymatic activity prevents the one electron reduction of quinones that results in the production of radical species. Mutations in this gene have been associated with tardive dyskinesia (TD), an increased risk of hematotoxicity after exposure to benzene, and susceptibility to various forms of cancer. Altered expression of this protein has been seen in many tumors and is also associated with Alzheimer's disease (AD). Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol NQO1
Entrez
OMIM
Uniprot