NPRA/NPR1 Antibody (377018) [Unconjugated]

NPRA/NPR1 was detected in immersion fixed paraffin-embedded sections of human kidney using Mouse Anti-Human NPRA/NPR1 Monoclonal Antibody (Catalog # MAB48601) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to epithelial cell membranes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

• Reactivity: Hu
• Applications: IHC
• Clone: 377018
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

NPRA/NPR1 Antibody (377018) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human NPR1/NPRA
  Gly33-Glu473
  Accession # P16066
• Specificity: Detects human NPR1/NPRA in direct ELISAs.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for NPRA/NPR1 Antibody (377018) [Unconjugated]

• ANPa
• ANP-A
• ANPRA
• ANPR-A
• ANPRAEC 4.6.1.2
• atrial natriuretic peptide receptor 1
• Atrial natriuretic peptide receptor type A
• atrionatriuretic peptide receptor A
• GC-A
• Guanylate cyclase A
• GUC2A
• GUCY2A
• natriuretic peptide A type receptor
• natriuretic peptide receptor A
• natriuretic peptide receptor A/guanylate cyclase A (atrionatriuretic peptidereceptor A)
• NPR1
• NPRA
• NPR-A
• Pndr

Background

Natriuretic peptide receptor A/guanylate cyclase A (NPR1), also called NPRA or GC-A, is a 120‑140 kDa type I transmembrane glycoprotein that is the primary receptor for natrietic peptides ANP and BNP. Binding of ANP to the extracellular ligand binding domain (aa 54‑415), plus ATP to the intracellular kinase homology domain (aa 528‑805) activates a cytoplasmic guanylate cyclase (aa 840‑1023). NPR1 is expressed most highly in kidney, adrenal and adipose tissue. Human NPR1 extracellular domain shows 86%, 44% and 34% aa identity with mouse NPR1, human NPRB and human NPRC, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NPRA/NPR1 Antibody (MAB48601)(1)

Publication using MAB48601

• Ma, X;Iyer, SR;Ma, X;Reginauld, SH;Chen, Y;Pan, S;Zheng, Y;Moroni, DG;Yu, Y;Zhang, L;Cannone, V;Chen, HH;Ferrario, CM;Sangaralingham, SJ;Burnett, JC; Evidence for Angiotensin II as a Naturally Existing Suppressor for the Guanylyl Cyclase A Receptor and Cyclic GMP Generation International journal of molecular sciences 2023-05-10 [PMID: 37239899] (Western Blot, Human)

Bioinformatics

• Uniprot:
  • P16066()