Nova1 Antibody

Western Blot: Nova1 Antibody [NBP1-00221] - HEK293 overexpressing NOVA1 and probed with (mock transfection in first lane).

Immunohistochemistry-Paraffin: Nova1 Antibody [NBP1-00221] - Staining of paraffin embedded Human Heart. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Western Blot: Nova1 Antibody [NBP1-00221] - Analysis of Nova1 in Human Breast Cancer lysate (35 ug protein in RIPA buffer) using NBP1-00221 at 0.01 ug/mL. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: Nova1 Antibody [NBP1-00221] - Staining of paraffin embedded Human Cerebral Cortex at 2.5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Immunohistochemistry-Paraffin: Nova1 Antibody [NBP1-00221] - Staining of paraffin embedded Human Cortex. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu
• Applications: WB, IHC, IHC-P, PEP-ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Nova1 Antibody Summary

• Immunogen: Peptide with sequence C-REMPQNVAKTEPVS corresponding to internal region according to NP_002506.2 , NP_006480.2 , NP_006482.1.
• Specificity: This antibody is expected to recognise all three reported isoforms (NP_002506.2 ; NP_006480.2 ; NP_006482.1)
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: NOVA1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5 ug/mL
  • Immunohistochemistry-Paraffin 5 ug/mL
  • Peptide ELISA Detection limit 1:64000
  • Western Blot 0.01 - 0.03 ug/mL
• Application Notes: WB: In transfected HEK293 transiently expressing NOVA1 a band of approx. 52 kDa is observed. This band is not observed in the non-transfected HEK293. IHC-P: Human cerebral cortex shows staining of selected nuclei.
• Control:

  Nova1 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Nova1 Antibody

• neuro-oncological ventral antigen 1Nova-1
• Onconeural ventral antigen 1
• Paraneoplastic Ri antigen
• RNA-binding protein Nova-1
• Ventral neuron-specific protein 1

Background

This gene encodes a neuron-specific RNA-binding protein, a member of the Nova family of paraneoplastic disease antigens, that is recognized and inhibited by paraneoplastic antibodies. These antibodies are found in the sera of patients with paraneoplastic opsoclonus-ataxia, breast cancer, and small cell lung cancer. Alternatively spliced transcripts encoding distinct isoforms have been described. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Nova1 Antibody (NBP1-00221)(1)

Publication using NBP1-00221

• Jelen N, Ule J, Zivin M, Darnell RB. Evolution of Nova-dependent splicing regulation in the brain. PLoS Genet 2007-10-12 [PMID: 17937501]

Bioinformatics

• Gene Symbol: NOVA1
• Entrez:
  • 4857(Human)
• Uniprot:
  • NP_002506.2(Human)