Nova1 Antibody

Images

 
Western Blot: Nova1 Antibody [NBP1-00221] - HEK293 overexpressing NOVA1 and probed with (mock transfection in first lane).
Immunohistochemistry-Paraffin: Nova1 Antibody [NBP1-00221] - Staining of paraffin embedded Human Heart. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Western Blot: Nova1 Antibody [NBP1-00221] - Analysis of Nova1 in Human Breast Cancer lysate (35 ug protein in RIPA buffer) using NBP1-00221 at 0.01 ug/mL. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: Nova1 Antibody [NBP1-00221] - Staining of paraffin embedded Human Cerebral Cortex at 2.5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunohistochemistry-Paraffin: Nova1 Antibody [NBP1-00221] - Staining of paraffin embedded Human Cortex. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

Nova1 Antibody Summary

Immunogen
Peptide with sequence C-REMPQNVAKTEPVS corresponding to internal region according to NP_002506.2, NP_006480.2, NP_006482.1.
Specificity
This antibody is expected to recognise all three reported isoforms (NP_002506.2 ; NP_006480.2 ; NP_006482.1)
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
NOVA1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.01 - 0.03 ug/mL
  • Immunohistochemistry 5 ug/mL
  • Immunohistochemistry-Paraffin 5 ug/mL
  • Peptide ELISA Detection limit 1:64000
Application Notes
WB: In transfected HEK293 transiently expressing NOVA1 a band of approx. 52 kDa is observed. This band is not observed in the non-transfected HEK293. IHC-P: Human cerebral cortex shows staining of selected nuclei.
Control
Nova1 Overexpression Lysate
Publications
Read Publication using NBP1-00221.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Nova1 Antibody

  • neuro-oncological ventral antigen 1Nova-1
  • Onconeural ventral antigen 1
  • Paraneoplastic Ri antigen
  • RNA-binding protein Nova-1
  • Ventral neuron-specific protein 1

Background

This gene encodes a neuron-specific RNA-binding protein, a member of the Nova family of paraneoplastic disease antigens, that is recognized and inhibited by paraneoplastic antibodies. These antibodies are found in the sera of patients with paraneoplastic opsoclonus-ataxia, breast cancer, and small cell lung cancer. Alternatively spliced transcripts encoding distinct isoforms have been described. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Nova1 Antibody (NBP1-00221)(1)


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Product General Protocols

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Video Protocols

WB Video Protocol

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

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Contact Information

Learn the difference between western blot and simple western

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Calculators

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Bioinformatics

Gene Symbol NOVA1
Entrez
Uniprot