Recombinant Human NOV/CCN3 Protein

• Reactivity: Hu
• Applications: PAGE, Bioactivity
• Format: Carrier-Free
• Concentration: LYOPH

Recombinant Human NOV/CCN3 Protein Summary

• Description: A single non-glycosylated polypeptide chain containing 331 amino acids corresponding to NOV/CCN3 Source: E. coli

  Uniprot ID: P48745

  Amino Acid Sequence: MQVAATQRCP PQCPGRCPAT PPTCAPGVRA VLDGCSCCLV CARQRGESCS DLEPCDESSG LYCDRSADPS NQTGICTAVE GDNCVFDGVI YRSGEKFQPS CKFQCTCRDG QIGCVPRCQL DVLLPEPNCP APRKVEVPGE CCEKWICGPD EEDSLGGLTL AAYRPEATLG VEVSDSSVNC IEQTTEWTAC SKSCGMGFST RVTNRNRQCE MLKQTRLCMV RPCEQEPEQP TDKKGKKCLR TKKSLKAIHL QFKNCTSLHT YKPRFCGVCS DGRCCTPHNT KTIQAEFQCS PGQIVKKPVM VIGTCTCHTN CPKNNEAFLQ ELELKTTRGK M

• Details of Functionality: CCN3 Protein is fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 1.0 ug/ml, corresponding to a specific activity of > 1000 IU/mg.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: CCN3
• Purity: >95%, by SDS-PAGE and HPLC
• Endotoxin Note: Less than 0.1 EU/ug of NOV/CCN3 as determined by LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Theoretical MW: 36.2 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered concentrated solution in 20 mM Tris-HCl, pH 8.6, 150 mM NaCl.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: >95%, by SDS-PAGE and HPLC
• Reconstitution Instructions: Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.

Alternate Names for Recombinant Human NOV/CCN3 Protein

• CCN family member 3
• CCN3
• CCN3IGF-binding protein 9
• IBP-9
• IGFBP-9
• IGFBP9novH
• Insulin-like growth factor-binding protein 9
• nephroblastoma overexpressed gene
• Nephroblastoma-overexpressed gene protein homolog
• NOV
• NOVH
• protein NOV homolog

Background

CCN3, also named NOV, IGFBP-9 and NovH, is belonging to the CCN (CTGF/CYR61/NOV) family and it is encoded by the NOV gene. It is expressed in certain tumors, including Wilm's tumor and most nephroblastomas. CCN3 (NOV) interacts with several proteins and is involved in both internal and external cell signaling. CCN3 (NOV) plays an important role in reducing tumorgenicity and proliferation of certain cancer cell lines. It also been reported to exert proangiogenic activities. Recombinant human CCN3 (NOV) is a 36.2 kDa protein containing 331 amino acid residues and it shares 80% and 81% a.a. identity with rat and murine NOV.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Publications for NOV/CCN3 Protein (NBP2-35084)(1)

Publication using NBP2-35084

• Babey ME, Krause WC, Herber CB et al. Brain-Derived CCN3 Is An Osteoanabolic Hormone That Sustains Bone in Lactating Females bioRxiv : the preprint server for biology 2023-08-29 [PMID: 37693376] (CellDiff)

FAQs for NOV/CCN3 Protein (NBP2-35084). (Showing 1 - 1 of 1 FAQ).

1. I am looking for the expression of CCN3/NOV in tumor cells and I am interested in finding an antibody that has a lot of data to support the antibody is working well. Can you make a recommendation?
   • We have two antibodies, NBP1-88154 and NBP1-88155 that have been extremely well characterized by testing at the Human Protein Atlas. Please see the Human Protein Atlas website for full testing for these products.

Bioinformatics

• Gene Symbol: CCN3
• Uniprot:
  • P48745()
  • P48745()
  • P48745()