NOA1 Overexpression Lysate (Native)

Images

 
Western Blot: NOA1 Overexpression Lysate (Adult Normal) [NBL1-08461] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for NOA1.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

NOA1 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC201000 and based on accession number NM_032313. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens chromosome 4 open reading frame 14 (C4orf14), mRNA.
Gene
NOA1

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
78.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for NOA1

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for NOA1 Overexpression Lysate (Native)

  • chromosome 4 open reading frame 14
  • hAtNOS1
  • mAtNOS1
  • MGC3232
  • mitochondrial nitric oxide synthase
  • nitric oxide synthase, mitochondrial (putative)
  • putative ortholog of Arabidopsis mitochondrial nitric oxide synthase

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-83761
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NB100-1585
Species: Hu
Applications: WB (-), ICC/IF, IHC-P, IP, PLA
NB100-1032
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P, PEP-ELISA
NB100-858
Species: Hu, Mu, Rt, GP, Mk
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P, PEP-ELISA, IHC-WhMt
NB100-56503
Species: Hu, Mu, Rt, Po, Ca, Dr, Eq, Ma, Rb
Applications: WB, Flow, ICC/IF, IHC, IHC-P
NB300-605
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-14871
Species: Hu, Mu
Applications: WB, ICC/IF
NBP1-26612
Species: Hu
Applications: IP (-), WB
NBP1-87283
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NB120-2860
Species: Hu, Mu, Rt, Ba, Bv, Ch
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P, ICC, IF
NBP1-98474
Species: Hu
Applications: WB
NBP1-83875
Species: Hu
Applications: WB, IHC, IHC-P
NBP1-92354
Species: Hu
Applications: IHC, IHC-P
NBP2-27084
Species: Hu, Pm
Applications: WB, IHC-P
H00057410-M02
Species: Hu, Mu, Rt
Applications: WB, ELISA
NBP1-87315
Species: Hu
Applications: IHC, IHC-P
NB100-2832
Species: Hu
Applications: WB, IHC, IHC-P, PEP-ELISA
NBP1-47836
Species: Hu
Applications: WB, Flow, IHC, IHC-P

Publications for NOA1 Lysate (NBL1-08461) (0)

There are no publications for NOA1 Lysate (NBL1-08461).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for NOA1 Lysate (NBL1-08461) (0)

There are no reviews for NOA1 Lysate (NBL1-08461). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for NOA1 Lysate (NBL1-08461) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional NOA1 Products

Bioinformatics Tool for NOA1 Lysate (NBL1-08461)

Discover related pathways, diseases and genes to NOA1 Lysate (NBL1-08461). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for NOA1 Lysate (NBL1-08461)

Discover more about diseases related to NOA1 Lysate (NBL1-08461).
 

Pathways for NOA1 Lysate (NBL1-08461)

View related products by pathway.

PTMs for NOA1 Lysate (NBL1-08461)

Learn more about PTMs related to NOA1 Lysate (NBL1-08461).

Blogs on NOA1

There are no specific blogs for NOA1, but you can read our latest blog posts.

Customers Who Bought This Also Bought

NOA1 Antibody
NBP1-81773
iNOS Antibody
NB300-605

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our NOA1 Overexpression Lysate (Native) and receive a gift card or discount.

Bioinformatics

Gene Symbol NOA1