NMNAT-1 Overexpression Lysate (Denatured)

NMNAT1 transfected lysate.

Lane 1: NMNAT1 transfected lysate (30.8 KDa) Lane 2: Non-transfected lysate.

• Reactivity: Hu
• Applications: WB

NMNAT-1 Overexpression Lysate (Denatured) Summary

• Description: Plasmid: pCMV-NMNAT1 full-length
• Gene: NMNAT1

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, and 0.01% Bromophenol blue).

Lysate Details for NMNAT-1

• Type: Overexpression
• Protein State: Denatured

Notes

Quality control test: Transient overexpression cell lysate was tested with Anti-NMNAT1 antibody by Western Blots.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for NMNAT-1 Overexpression Lysate (Denatured)

• EC 2.7.7.1
• EC 2.7.7.18
• NaMN adenylyltransferase 1
• nicotinamide mononucleotide adenylyltransferase 1
• nicotinamide nucleotide adenylyltransferase 1
• nicotinamide nucleotide adenylyltransferase
• Nicotinate-nucleotide adenylyltransferase 1
• NMN adenylyltransferase 1
• NMNAT1
• NMNAT-1
• NMNATPNAT1
• PNAT1
• pyridine nucleotide adenylyltransferase 1

Background

The coenzyme NAD and its derivatives are involved in hundreds of metabolic redox reactions and are utilized in protein ADP-ribosylation, histone deacetylation, and in some Ca(2+) signaling pathways. NMNAT (EC 2.7.7.1) is a central enzyme in NAD biosynthesis, catalyzing the condensation of nicotinamide mononucleotide (NMN) or nicotinic acid mononucleotide (NaMN) with the AMP moiety of ATP to form NAD or NaAD (Zhang et al., 2003 [PubMed 12574164]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

FAQs for NMNAT-1 Lysate (H00064802-T01). (Showing 1 - 1 of 1 FAQ).

1. I am interested in using this product as an enzyme, and before ordering I would like to know if it is enzymatically active?
   • In regards to your inquiry about the lysates and protein we sell, unless otherwise noted on the datasheet these products are not considered active. The lysates will be transfected with the protein sequence and do not always undergo the same post translational modifications and folding as an endogenous protein. Unless specifically stated we cannot guarantee activity. These lysates are mainly intended as positive controls in Western Blot.

Bioinformatics

• Gene Symbol: NMNAT1
• Entrez:
  • 64802(Human)