NMNAT-1 Overexpression Lysate (Denatured) Summary
Description |
Quality control test: Transient overexpression cell lysate was tested with Anti-NMNAT1 antibody by Western Blots. Plasmid: pCMV-NMNAT1 full-length |
Gene |
NMNAT1 |
Applications/Dilutions
Packaging, Storage & Formulations
Storage |
Store at -80C. Avoid freeze-thaw cycles. |
Buffer |
Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, and 0.01% Bromophenol blue). |
Lysate Details for Array
Type |
Overexpression |
Protein State |
Denatured |
Notes
This product is produced by and distributed for Abnova, a company based in Taiwan.
Alternate Names for NMNAT-1 Overexpression Lysate (Denatured)
Background
The coenzyme NAD and its derivatives are involved in hundreds of metabolic redox reactions and are utilized in protein ADP-ribosylation, histone deacetylation, and in some Ca(2+) signaling pathways. NMNAT (EC 2.7.7.1) is a central enzyme in NAD biosynthesis, catalyzing the condensation of nicotinamide mononucleotide (NMN) or nicotinic acid mononucleotide (NaMN) with the AMP moiety of ATP to form NAD or NaAD (Zhang et al., 2003 [PubMed 12574164]).[supplied by OMIM]
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are
guaranteed for 6 months from date of receipt.
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Product General Protocols
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Video Protocols
FAQs for NMNAT-1 Lysate (H00064802-T01). (Showing 1 - 1 of 1 FAQ).
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I am interested in using this product as an enzyme, and before ordering I would like to know if it is enzymatically active?
- In regards to your inquiry about the lysates and protein we sell, unless otherwise noted on the datasheet these products are not considered active. The lysates will be transfected with the protein sequence and do not always undergo the same post translational modifications and folding as an endogenous protein. Unless specifically stated we cannot guarantee activity. These lysates are mainly intended as positive controls in Western Blot.
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