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NMNAT-1 Antibody (OTI1G4) - Azide and BSA Free

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Western Blot: NMNAT-1 Antibody (OTI1G4) - Azide and BSA Free [NBP2-73017] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Nmnat1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Nmnat1.
Western Blot: NMNAT-1 Antibody (OTI1G4) - Azide and BSA Free [NBP2-73017] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Nmnat1 (Right lane) cDNA for 48 hrs and lysed. ...read more
Immunohistochemistry: NMNAT-1 Antibody (OTI1G4) - Azide and BSA Free [NBP2-73017] - Staining of paraffin-embedded Human pancreas tissue using anti-Nmnat1 mouse monoclonal antibody.
Flow Cytometry: NMNAT-1 Antibody (OTI1G4) - Azide and BSA Free [NBP2-73017] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-Nmnat1 ...read more
Western Blot: NMNAT-1 Antibody (OTI1G4) - Azide and BSA Free [NBP2-73017] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Nmnat1 monoclonal antibody.
Immunohistochemistry: NMNAT-1 Antibody (OTI1G4) - Azide and BSA Free [NBP2-73017] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-Nmnat1 mouse monoclonal antibody.
Immunohistochemistry: NMNAT-1 Antibody (OTI1G4) - Azide and BSA Free [NBP2-73017] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-Nmnat1 mouse monoclonal antibody.
Immunohistochemistry: NMNAT-1 Antibody (OTI1G4) - Azide and BSA Free [NBP2-73017] - Staining of paraffin-embedded Human endometrium tissue using anti-Nmnat1 mouse monoclonal antibody.
Immunohistochemistry: NMNAT-1 Antibody (OTI1G4) - Azide and BSA Free [NBP2-73017] - Staining of paraffin-embedded Human Kidney tissue using anti-Nmnat1 mouse monoclonal antibody.
Immunohistochemistry: NMNAT-1 Antibody (OTI1G4) - Azide and BSA Free [NBP2-73017] - Staining of paraffin-embedded Human Ovary tissue using anti-Nmnat1 mouse monoclonal antibody.
Immunohistochemistry: NMNAT-1 Antibody (OTI1G4) - Azide and BSA Free [NBP2-73017] - Staining of paraffin-embedded Human prostate tissue using anti-Nmnat1 mouse monoclonal antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, IHC, CyTOF-ready
Clone
OTI1G4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-73017AF350
Alexa Fluor 405
NBP2-73017AF405
Alexa Fluor 488
NBP2-73017AF488
Alexa Fluor 532
NBP2-73017AF532
Alexa Fluor 594
NBP2-73017AF594
Alexa Fluor 647
NBP2-73017AF647
Alexa Fluor 700
NBP2-73017AF700
Alexa Fluor 750
NBP2-73017AF750
Allophycocyanin
NBP2-73017APC
Allophycocyanin/Cy7
NBP2-73017APCCY7
Biotin
NBP2-73017B
DyLight 350
NBP2-73017UV
DyLight 405
NBP2-73017V
DyLight 488
NBP2-73017G
DyLight 550
NBP2-73017R
DyLight 594
NBP2-73017DL594
DyLight 650
NBP2-73017C
DyLight 680
NBP2-73017FR
DyLight 755
NBP2-73017IR
FITC
NBP2-73017F
HRP
NBP2-73017H
Janelia Fluor 549
NBP2-73017JF549
Janelia Fluor 646
NBP2-73017JF646
PE
NBP2-73017PE
PE/Atto594
NBP2-73017PEATT594
PE/Cy5.5
NBP2-73017PECY55
PE/Cy7
NBP2-73017PECY7
PerCP
NBP2-73017PCP
Unconjugated
NBP2-03452
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-03452
100% Guarantee on every product

NMNAT-1 Antibody (OTI1G4) - Azide and BSA Free Summary

Immunogen
Full length human recombinant protein of human NMNAT1 (NP_073624) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
NMNAT1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot 1:500-2000
  • Flow Cytometry 1:100
  • Immunohistochemistry 1:150
  • CyTOF-ready
Theoretical MW
31.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute with PBS (pH 7.3). To use this carrier-free antibody for conjugation experiments, another round of desalting is highly recommended.

Alternate Names for NMNAT-1 Antibody (OTI1G4) - Azide and BSA Free

  • EC 2.7.7.1
  • EC 2.7.7.18
  • NaMN adenylyltransferase 1
  • nicotinamide mononucleotide adenylyltransferase 1
  • nicotinamide nucleotide adenylyltransferase 1
  • nicotinamide nucleotide adenylyltransferase
  • Nicotinate-nucleotide adenylyltransferase 1
  • NMN adenylyltransferase 1
  • NMNAT1
  • NMNAT-1
  • NMNATPNAT1
  • PNAT1
  • pyridine nucleotide adenylyltransferase 1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Immunocytochemistry/Immunofluorescence: UBE4B Antibody [NBP1-32737] - Analysis of methanol-fixed Hep3B, using antibody at 1:500 dilution.
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Western Blot: Sirtuin 1/SIRT1 Antibody (1F3) [NBP1-51641] - Western blot analysis using SIRT1 mouse mAb against MCF-7 (1), Jurkat (2), Hela (3), HEK293 (4) and A549 (5) cell lysates.
Immunocytochemistry/Immunofluorescence: Sirtuin 1/SIRT1 Antibody (1F3) [NBP1-51641] - SIRT1 antibody was tested at 1:10 in Ntera2 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). Image objective 40x.
Immunohistochemistry-Paraffin: Sirtuin 1/SIRT1 Antibody (1F3) [NBP1-51641] - Immunohistochemical analysis of paraffin-embedded lung cancer tissues (left) and kidney cancer tissues (right) using SIRT1 mouse mAb with DAB staining.
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PBEF/Visfatin/NAMPT An
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Western Blot: PBEF/Visfatin/NAMPT Antibody [NBP2-23795] - DETECTS A STRONG BAND AROUND 37Kda AND ALSO THE OTHER ISOFORMS BETWEEN 40 AND 55Kda. THE DILUTION OF THE ANTIBODY CAN BE ENHANCED 1:5000. Image from a verified customer review.
Western Blot: PBEF/Visfatin/NAMPT Antibody [NBP2-23795] - NAMPT Antibody [NBP2-23795] - Analysis of NAMPT in human tumor cell line INSTA-Blot at 1:1000. Lanes: (A) HeLa, (B) Jurkat, (C) Daudi, (D) HEK 293, (E) Rh30, (F) A375, (G) T98G, (H) HCT-116, (I) Hep-2.
Western Blot: PBEF/Visfatin/NAMPT Antibody [NBP2-23795] - NAMPT Antibody [NBP2-23795] - Western blot analysis of NAMPT in multiple samples using NBP2-23795 at 1:1000. Lanes: (1) Human spleen, (2) Human spleen in the presence of immunizing/blocking peptide, (3) HeLa, (4) Jurkat, (5) Rh30, (6) T98G, (7) HCT-116.
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Western Blot: p97/VCP Antibody [NB100-1558] - Zymographic assay of gelatinase activity. Medium supernatants of 20ml culture medium (medium control) as well as 20 ml conditioned medium from 107 U937 cells, pMTH1-U937 cells and asCD11b-U937 cells in the absence or presence of 5nM TPA for 72 h, respectively, were 18-fold concentrated and subjected to SDS-PAGE containing 2 mg/ml of gelatine. Following incubation with MMP enzyme buffer, the gels were stained with 0.4% Coomassie blue and afterwards destained again to visualize the appearance of gelatinase activity by light bands against the dark background. The molecular weight markers on both sides of the gels indicate the size of the MMPs exhibiting gelatinase activities. Image collected and cropped by CiteAb from the following publication (http://biosignaling.biomedcentral.com/articles/10.1186/1478-811X-10-13) licensed under a CC-BY licence.
Western Blot: p97/VCP Antibody [NB100-1558] - Whole cell lysate (15 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit antiVCP antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.
Western Blot: p97/VCP Antibody [NB100-1558] - Detection of human VCP by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-VCP antibody NB100-1558 used for IP at 3 ug per reaction. VCP was also immunoprecipitated by rabbit anti-VCP antibody NB100-1557. For blotting immunoprecipitated VCP, NB100-1558 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.
(10 Publications)
Species: Hu, Mu, Rt, Ze
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PARP Antibody
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NB100-56599
Western Blot: PARP Antibody (194C1439) - Cleaved [NB100-56599] - Analysis of cleaved PARP in staurosporine-treated Jurkat cells at various time points, antibody at 2 ug/mL. The band corresponding to cleaved PARP is only seen in the treated samples. Anti-mouse Ig HRP conjugate was used in this test.
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Flow Cytometry: DAP5 Antibody (39C534.1) [NB120-11929] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and HEK-293 (B) cells using antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.
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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-73017AF350
Alexa Fluor 405 NBP2-73017AF405
Alexa Fluor 488 NBP2-73017AF488
Alexa Fluor 532 NBP2-73017AF532
Alexa Fluor 594 NBP2-73017AF594
Alexa Fluor 647 NBP2-73017AF647
Alexa Fluor 700 NBP2-73017AF700
Alexa Fluor 750 NBP2-73017AF750
Allophycocyanin NBP2-73017APC
Allophycocyanin/Cy7 NBP2-73017APCCY7
Biotin NBP2-73017B
DyLight 350 NBP2-73017UV
DyLight 405 NBP2-73017V
DyLight 488 NBP2-73017G
DyLight 550 NBP2-73017R
DyLight 594 NBP2-73017DL594
DyLight 650 NBP2-73017C
DyLight 680 NBP2-73017FR
DyLight 755 NBP2-73017IR
FITC NBP2-73017F
HRP NBP2-73017H
Janelia Fluor 549 NBP2-73017JF549
Janelia Fluor 646 NBP2-73017JF646
PE NBP2-73017PE
PE/Atto594 NBP2-73017PEATT594
PE/Cy5.5 NBP2-73017PECY55
PE/Cy7 NBP2-73017PECY7
PerCP NBP2-73017PCP
Learn about Custom Antibody Labeling

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Bioinformatics

Gene Symbol NMNAT1