NKIRAS1 Overexpression Lysate

Images

 
Western Blot: NKIRAS1 Overexpression Lysate (Adult Normal) [NBL1-13655] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for NKIRAS1.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

NKIRAS1 Overexpression Lysate Summary

Description
Transient overexpression lysate of NFKB inhibitor interacting Ras-like 1 (NKIRAS1) The lysate was created in HEK293T cells, using Plasmid ID RC204248 and based on accession number NM_020345. The protein contains a C-MYC/DDK Tag.
Gene
NKIRAS1

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot.

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
Theoretical MW
21.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for NKIRAS1 Overexpression Lysate

  • I-kappa-B-interacting Ras-like protein 1
  • kappa B-ras 1
  • Kappa B-Ras protein 1
  • KappaB-Ras1
  • KBRAS1kappaB-Ras1
  • NF-kappa-B inhibitor-interacting Ras-like protein 1
  • NFKB inhibitor interacting Ras-like 1
  • NFKB inhibitor interacting Ras-like protein 1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB100-56583
Species: Hu, Rb
Applications: WB, Flow, IHC, IHC-P, KO
NBP1-53169
Species: Hu
Applications: WB, IHC, IHC-P
H00028511-M02
Species: Hu
Applications: WB, ELISA, IHC, IHC-P
NB100-65125
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, CyTOF-ready
NBP2-16521
Species: Hu, Mu, Sq
Applications: WB, ChIP, ICC/IF, IHC, IHC-P, IP
1670-SE
Species: Ec
AF3827
Species: Mu
Applications: WB, Flow, CyTOF-ready
NBP2-20212
Species: Hu
Applications: WB, IHC, IHC-P
MAB3425
Species: Hu, Rt
Applications: WB
NB100-56507
Species: Hu, Mu, Rt
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-P, IP, CyTOF-ready, KO
NBP1-84528
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
AF3235
Species: Hu, Mu, Rt
Applications: WB, IHC
NBP1-81480
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NB600-247
Species: Hu
Applications: WB
MAB7635
Species: Hu
Applications: WB
H00084107-M09
Species: Hu
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
NBL1-13655
Species: Hu
Applications: WB

Publications for NKIRAS1 Lysate (NBL1-13655) (0)

There are no publications for NKIRAS1 Lysate (NBL1-13655).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for NKIRAS1 Lysate (NBL1-13655) (0)

There are no reviews for NKIRAS1 Lysate (NBL1-13655). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for NKIRAS1 Lysate (NBL1-13655) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Other Available Formats

NBL1-13655

Additional NKIRAS1 Products

Bioinformatics Tool for NKIRAS1 Lysate (NBL1-13655)

Discover related pathways, diseases and genes to NKIRAS1 Lysate (NBL1-13655). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for NKIRAS1 Lysate (NBL1-13655)

Discover more about diseases related to NKIRAS1 Lysate (NBL1-13655).
 

Pathways for NKIRAS1 Lysate (NBL1-13655)

View related products by pathway.

PTMs for NKIRAS1 Lysate (NBL1-13655)

Learn more about PTMs related to NKIRAS1 Lysate (NBL1-13655).
 

Research Areas for NKIRAS1 Lysate (NBL1-13655)

Find related products by research area.

Blogs on NKIRAS1

There are no specific blogs for NKIRAS1, but you can read our latest blog posts.
Recombinant Monoclonal Antibodies

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our NKIRAS1 Overexpression Lysate and receive a gift card or discount.

Bioinformatics

Gene Symbol NKIRAS1
COVID-19 update