NKIRAS1 Antibody

Product Discontinued

NKIRAS1 Antibody Summary

• Immunogen: Antibody was raised against a 15 amino acid synthetic peptide from near the carboxy terminus of human KappaB ras1. The immunogen is located within the last 50 amino acids of KappaB ras1.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NKIRAS1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1:100-1:2000
• Application Notes: This antibody can be used for detection of KappaB ras1 by Western blot at 0.5 - 1 mg/ml.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for NKIRAS1 Antibody

• I-kappa-B-interacting Ras-like protein 1
• kappa B-ras 1
• Kappa B-Ras protein 1
• KappaB-Ras1
• KBRAS1kappaB-Ras1
• NF-kappa-B inhibitor-interacting Ras-like protein 1
• NFKB inhibitor interacting Ras-like 1
• NFKB inhibitor interacting Ras-like protein 1

Background

KappaB ras-1 (kappaB-ras-1) and kappaB-ras-2 are two small proteins that similar to Ras-like small GTPases that associate with IkappaB (IkappaB), an inhibitor of the transcription factor NF-kappaB. IkappaB exists in two homologous forms, IkappaB-alpha and IkappaB-beta, although IkappaB-beta contains a unique 47-amino acid region within its ankyrin domain. While inactive IkappaB-alpha-NF-kappaB complexes can shuttle in and out of the nucleus, IkappaB-beta-NF-kappaB complexes are retained exclusively in the cytoplasm. It is suggested that kappaB-ras proteins preferentially bind to the IkappaB-beta form through this unique insert within the ankyrin region, thus modulating the cellular location of IkappaB-beta and regulating the rate of degradation of IkappaB-beta. This antibody is specific for kappaB-ras1 and has no cross-reactivity to kappaB-ras2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NKIRAS1
• Uniprot:
  • AAF34998(Human)