NIRF Overexpression Lysate (Native)

Images

 
Western Blot: NIRF Overexpression Lysate (Adult Normal) [NBL1-17604] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for NIRF.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

NIRF Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC219579 and based on accession number NM_152896. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens ubiquitin-like with PHD and ring finger domains 2 (UHRF2), mRNA.
Gene
UHRF2

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
89.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for NIRF Overexpression Lysate (Native)

  • DKFZp434B0920
  • DKFZp686G0837
  • E3 ubiquitin-protein ligase UHRF2
  • EC 6.3.2
  • EC 6.3.2.-
  • MGC33463
  • NIRFUbiquitin-like-containing PHD and RING finger domains protein 2
  • Np95/ICBP90-like RING finger protein
  • Np95-like RING finger protein
  • Nuclear protein 97
  • Nuclear zinc finger protein Np97
  • RNF107RING finger protein 107
  • Ubiquitin-like PHD and RING finger domain-containing protein 2
  • ubiquitin-like with PHD and ring finger domains 2
  • ubiquitin-like, containing PHD and RING finger domains, 2
  • URF2

Background

This gene encodes a nuclear protein which is involved in cell-cycle regulation. The encoded protein is a ubiquitin-ligase capable of ubiquinating PCNP (PEST-containing nuclear protein), and together they may play a role in tumorigenesis. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Video Protocols

WB Video Protocol

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Bioinformatics

Gene Symbol UHRF2