NIRF Overexpression Lysate (Native)


Western Blot: NIRF Overexpression Lysate (Adult Normal) [NBL1-17604] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for NIRF.

Product Details

Reactivity HuSpecies Glossary
Applications WB

Order Details

NIRF Overexpression Lysate (Native) Summary

The lysate was created in HEK293T cells, using plasmid ID RC219579 and based on accession number NM_152896. The protein contains a C-terminal DDK tag.
Homo sapiens ubiquitin-like with PHD and ring finger domains 2 (UHRF2), mRNA.


Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
89.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Store at -80C. Avoid freeze-thaw cycles.
RIPA buffer

Lysate Details for Array

Protein State


HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for NIRF Overexpression Lysate (Native)

  • DKFZp434B0920
  • DKFZp686G0837
  • E3 ubiquitin-protein ligase UHRF2
  • EC 6.3.2
  • EC 6.3.2.-
  • MGC33463
  • NIRFUbiquitin-like-containing PHD and RING finger domains protein 2
  • Np95/ICBP90-like RING finger protein
  • Np95-like RING finger protein
  • Nuclear protein 97
  • Nuclear zinc finger protein Np97
  • RNF107RING finger protein 107
  • Ubiquitin-like PHD and RING finger domain-containing protein 2
  • ubiquitin-like with PHD and ring finger domains 2
  • ubiquitin-like, containing PHD and RING finger domains, 2
  • URF2


This gene encodes a nuclear protein which is involved in cell-cycle regulation. The encoded protein is a ubiquitin-ligase capable of ubiquinating PCNP (PEST-containing nuclear protein), and together they may play a role in tumorigenesis. [provided by RefSeq].


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

Species: Hu
Applications: WB, ELISA, Flow, ICC/IF, IHC
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu
Applications: WB, Simple Western, IHC, IP, KO
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu, Mu, Rt, Pm
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
Species: Mu
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
Species: Hu
Applications: WB, IHC, IHC-P
Species: Hu, Mu, Rt, Po, Bv, Ch, Eq
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
Species: Hu, Mu, Rt, Ye, Xp(-)
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, CyTOF-ready, Flow-IC
Species: Hu
Applications: ICC/IF, IHC, IHC-P
Species: Hu
Applications: WB, IHC, IHC-P
Species: Hu, Mu
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P, CyTOF-ready

Publications for NIRF Lysate (NBL1-17604) (0)

There are no publications for NIRF Lysate (NBL1-17604).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for NIRF Lysate (NBL1-17604) (0)

There are no reviews for NIRF Lysate (NBL1-17604). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for NIRF Lysate (NBL1-17604) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional Array Products

Bioinformatics Tool for NIRF Lysate (NBL1-17604)

Discover related pathways, diseases and genes to NIRF Lysate (NBL1-17604). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for NIRF Lysate (NBL1-17604)

Discover more about diseases related to NIRF Lysate (NBL1-17604).

Pathways for NIRF Lysate (NBL1-17604)

View related products by pathway.

PTMs for NIRF Lysate (NBL1-17604)

Learn more about PTMs related to NIRF Lysate (NBL1-17604).

Research Areas for NIRF Lysate (NBL1-17604)

Find related products by research area.

Blogs on NIRF

There are no specific blogs for NIRF, but you can read our latest blog posts.

Customers Who Bought This Also Bought

NIRF Antibody (3A11)
Albumin Antibody

Contact Information

Product PDFs


Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.


Review this Product

Be the first to review our NIRF Overexpression Lysate (Native) and receive a gift card or discount.


Gene Symbol UHRF2