NIR2 Antibody

Immunocytochemistry/Immunofluorescence: NIR2 Antibody [NB100-1417] - Immunofluorescence analysis of paraformaldehyde fixed A549 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic and Endoplasmic Reticulum staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Flow Cytometry: NIR2 Antibody [NB100-1417] - Flow cytometric analysis of paraformaldehyde fixed A549 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

• Reactivity: Mu, Hu, Bv, Ca
• Applications: ELISA, Flow, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

NIR2 Antibody Summary

• Immunogen: Peptide with sequence C-KARSISLKLDSEE corresponding to C-Terminus according to NP_004901.2, NP_001124320.1.
• Localization: Cytoplasmic
• Specificity: This antibody is expected to recognise both reported isoforms.
• Predicted Species: Human (100%), Canine (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PITPNM1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence 10 ug/ml
  • Peptide ELISA Detection limit 1:8000
• Application Notes: WB: An anonymous customer has reported a band at approx. 160 kDa band in lysates of a mouse Macrophage cell line after 2ug/ml antibody staining (calculated MW of 135 kDa band according to NP_004901.1). ICC: An anonymous customer has reported staining of vesicular cytoplasm in the same cell line.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for NIR2 Antibody

• DRES9NIR-2
• Drosophila retinal degeneration B homolog
• FLJ44997
• membrane-associated phosphatidylinositol transfer protein 1
• NIR2PITPNM
• phosphatidylinositol transfer protein, membrane-associated 1Pyk2 N-terminal domain-interacting receptor 2
• PITPnm 1
• Rd9
• RDGB
• RDGB1
• RDGBA
• RDGBA1
• retinal degeneration B alpha 1

Background

NIR2 belongs to a family of proteins that share homology with the Drosophila retinal degeneration B (rdgB) protein. Nir2, a human homolog of Drosophila melanogaster retinal degeneration B protein, is essential for cytokinesis. A specific Thr residue in the Nir2 PI-transfer domain provides a regulatory site for targeting to lipid droplets. This may affect intracellular lipid trafficking and distribution & explain the dominant effect of the RdgB-T59E mutant on retinal degeneration.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NIR2 Antibody (NB100-1417)(1)

Publication using NB100-1417

• Lev S, Hernandez J, Martinez R et al. Identification of a novel family of targets of PYK2 related to Drosophila retinal degeneration B (rdgB) protein. Mol Cell Biol 1999-03-01 [PMID: 10022914]

Bioinformatics

• Gene Symbol: PITPNM1
• Entrez:
  • 9600(Human)
  • 18739(Mouse)
• Uniprot:
  • NP_004901.(Human)